影响骨髓间充质干细胞成骨能力的相关物理因素及机制  

Physical factors and mechanisms affecting osteogenic ability of bone marrow mesenchymal stem cells

作　　者：崔鑫涛 张震宇[1] Cui Xintao;Zhang Zhenyu(First Affiliated Hospital of Harbin Medical University,Harbin 150001,Heilongjiang Province,China)

机构地区：[1]哈尔滨医科大学附属第一医院,黑龙江省哈尔滨市150001

出　　处：《中国组织工程研究》2020年第31期5064-5070,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:促进干细胞向成骨分化、诱导骨细胞增殖进而促进骨形成,被认为是治疗骨性疾病的重要研究方向,大量研究集中在寻找促进骨髓间充质干细胞成骨分化的诱导因素上,探讨骨髓间充质干细胞定向增殖和成骨分化的最佳诱导条件受到了越来越多研究者的关注。目的:综述并讨论相关物理因素对骨髓间充质干细胞增殖及定向成骨分化的影响及可能涉及的信号转导通路作用机制。方法:检索万方、中国期刊全文数据库(CNKI)、PubMed、GeenMedical等数据库2000年6月至2019年11月期间关于影响骨髓间充质干细胞成骨分化相关物理因素的文章,检索词为“骨髓间充质干细胞,成骨,分化”“bone marrow mesenchymal stem cells,osteogenesis differentiation”,排除陈旧以及重复的观点,将检索到的文献进行整理,纳入72篇文献进行分析。结果与结论:相关力学因素(如压应力、剪切应力、牵张应力、微重力、低频震动)及电磁场、超声波、电刺激、冷刺激等对骨髓间充质干细胞增殖及成骨能力均有一定的影响,并通过相关的因子及信号通路实现,但其作用受移植部位微环境差异的影响,并且某些物理因素还具有双重作用。在未来,寻找不同微环境下对骨髓间充质干细胞具有最佳诱导作用的影响因素以及这些因素发生作用的机制,将对干细胞的应用及组织工程的发展起到巨大的推动作用。BACKGROUND:To accelerate osteogenic differentiation,induce cell proliferation and in turn promote bone formation is considered to be an important research direction in the treatment of osseous diseases.Numerous studies have focused on looking for inducing factors that promote the osteogenic differentiation of bone marrow mesenchymal stem cells.To investigate the optimal conditions for inducing proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells has been an increasing concern.OBJECTIVE:To review and discuss the effects of physical factors related to the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells and the possible mechanisms of signal transduction pathways.METHODS:To search for articles on physical factors affecting osteogenic differentiation of bone marrow mesenchymal stem cell in WanFang,CNKI,PubMed,GeenMedical from June 2000 to November 2019,the search terms were“bone marrow mesenchymal stem cells,osteogenesis,differentiation”in Chinese and English,respectively.The old and repeated viewpoints were excluded,and finally 72 literatures were used for analysis.RESULTS AND CONCLUSION:Relevant mechanical factors(such as compressive stress,shear stress,stretch stress,microgravity,low-frequency vibration),electromagnetic field,ultrasonic wave,electrical stimulation,and cold stimulation,have certain effects on the proliferation and osteogenic ability of bone marrow mesenchymal stem cells,which are realized through relevant factors and signaling pathways.However,their effects are influenced by the microenvironment of the transplant site,and some physical factors show a dual effect.Future investigation on the influencing factors with the optimal induction of bone marrow mesenchymal stem cells under different microenvironments as well as the mechanism of these factors will greatly promote the application of stem cells and the development of tissue engineering.

关 键 词：骨髓间充质干细胞 成骨能力 物理因素 力学因素 干细胞 通路 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]