营养成分对‘安陆1号’银杏胚离体培养成苗的影响  

Effect of Nutrient Contents to Plant Regeneration in Vitro-culture from Embryos of Ginkgo biloba‘Anlu-1’

作　　者：胡燕梅 张力 郭云贵[2] 方中明[2,3] HU Yanmei;ZHANG Li;GUO Yungui;FANG Zhongming(Journal Department,Jianghan University,Wuhan 430056,China;School of Life Science and Technology,Wuhan University of Bioengineering,Wuhan 430415,China;College of Agriculture,Guizhou University,Guiyang 550025,China)

机构地区：[1]江汉大学期刊社,武汉430056 [2]武汉生物工程学院生命科学与技术学院,武汉430415 [3]贵州大学农学院,贵阳550025

出　　处：《湖北民族大学学报（自然科学版）》2020年第2期126-129,157,共5页Journal of Hubei Minzu University:Natural Science Edition

基　　金：国家自然科学基金项目(3130150);湖北省教育厅科学技术研究项目(B201539,B2015394).

摘　　要：以‘安陆1号’品种银杏胚为材料,探讨基本培养基及外源添加物对银杏胚离体萌发成苗的影响.结果表明:‘安陆1号’银杏胚在基本培养基N6中最易萌发成苗,其成苗率达到36.7%;N6中加入适宜添加物椰子乳、活性炭、水解酪蛋白和银杏胚乳汁均能更有效促进胚萌发和成苗;其中0.5 g/L水解酪蛋白的促进效果最好,银杏胚离体萌发的成苗率达到92.8%,平均株高达到4.1 cm,且幼苗健康粗壮,为‘安陆1号’银杏胚的离体萌发成苗提供了技术可能.Taking of Ginkgo biloba‘Anlu-1’as test materials,we discussed the effect of basic medium and appendages on the embryos ginkgo embryos of plantlet in vitro culture.The results show that the basic medium N6 was more appropriat for seedling and the seedling rate reached 36.7%;the suitable hydrolyzed casein and Ginkgo embryo milk added in basic medium N6 contributed to embryo germination and growth;For 0.5 g/L hydrolyzed casein test,seedling rate reached 92.8%,the average strain reached 4.1 cm and the seedling was sturdy.The culture in vitro system of Ginkgo biloba‘Anlu-1’was established throuth this experiment.

关 键 词：'安陆1号’银杏胚 离体培养 水解酪蛋白 胚乳汁 

分 类 号：S792.95[农业科学—林木遗传育种] Q813.12[农业科学—林学]