瘦素对2型糖尿病大鼠侧脑室交感神经活性和压力感受性反射功能的影响  

Effects of lateral ventricle infusion leptin on sympathetic activity and baroreceptor reflex in type 2 diabetic rats

作　　者：李宝新[1] 张云良[1] 董越华[1] 辛欢欢[1] 刘莉芳[1] 常文龙[1] 王翯[1] 郭淑芹[1] LI Baoxin;ZHANG Yunliang;DONG Yuehua(Department of Endocrinology,Baoding First Central Hospital,Baoding 071000,China)

机构地区：[1]保定市第一中心医院内分泌科,071000

出　　处：《中国糖尿病杂志》2020年第4期292-296,共5页Chinese Journal of Diabetes

基　　金：国家自然科学基金青年(81500644);河北省医学科学研究重点课题计划(20170230);保定市科学技术研究与发展计划(16ZF071)。

摘　　要：目的通过对T2DM大鼠进行侧脑室输注瘦素(LPN),探讨LPN对T2DM大鼠交感神经活性和压力感受性反射功能的影响。方法 20只SD大鼠随机分为正常对照(NC)组、糖尿病组(DM),每组各10只,采用高糖高脂饲料喂养联合STZ(30 mg/kg)腹腔注射制备T2DM模型。两组于麻醉下行2 h侧脑室LPN输注,并测定大鼠的交感神经活性(SNA)、平均动脉压(MAP)、心率(HR),绘制压力感受性反射曲线。结果 DM组1 hLPN和2 hLPN输注后的SNA较NC组[(162±19)%vs(145±21)%、(216±24)%vs(183±27)%]、MAP[(114±11)vs(104±7)、(125±14)vs(115±12)mmHg]、HR[(363±39)vs(342±36)、(441±47)vs(386±43)bpm]均升高(P<0.05);DM组1 hLPN和2 hLPN输注后交感神经压力反射功能参数Max较NC组[(279±27)%vs(249±25)%、(381±38)%vs(336±36)%]、Min[(94±12)%vs(66±8)%、(136±17)%vs(87±13)%]、BP50[(105±8)vs(98±7)、(115±11)vs(106±10)mmHg]升高(P<0.05)。结论 LPN可明显增加T2DM大鼠的交感神经活性,并对压力感受性反射功能有一定影响。Objective To investigate the effect of leptin on sympathetic nerve activity(SNA)and baroreflex function in rats with type 2 diabetes mellitus by lateral ventricle infusion.rats were randomly divided into normal control group(NC group)and diabetic group(DM group).Rats were fed with high sugar and high-fat diet combined with STZ(30 mg/kg)intraperitoneally to prepare DM model.Leptin was infused into the lateral ventricle for two hours after anesthesia.SNA,MAP and HR The basal SNA[(162±19)% vs(145±21)%,(216±24)% vs(183±27)%],MAP[(114±11)vs(104±7),(125±14)vs(115±12)mmHg]and HR[(363±39)vs(342±36),(441±47)vs(386±43)bpm]after 1 hLPN and 2 hLPN infusion in DM rats were significantly higher than those in NC rats(P<0.05).The sympathetic baroreflex parameters Max[(279±27)% vs(249±25)%,(381±38)% vs(336±36)%)],Min[(94±12)% vs(66±8)%,(136±17)% vs(87±13)%]and BP50[(105±8)vs(98±7),(115±11)vs(106±10)mmHg]in DM rats were significantly higher than those of NC rats after 1 h and 2 hLPN infusion(P<0.05).Conclusion Leptin can significantly increase the sympathetic nerve activity and exert a certain influence on the baroreflex function in type 2 diabetic rats.

关 键 词：瘦素 糖尿病 2型 交感神经活性 压力感受性反射功能 大鼠 

分 类 号：R587.1[医药卫生—内分泌] R544.1[医药卫生—内科学]