针刺对慢性束缚应激大鼠脑脊液载脂蛋白和视黄醇结合蛋白表达的影响  

Effects of acupuncture on the expression of apolipoprotein and retinol-binding protein in the cerebrospinal fluid of chronic restrained-stress model rats

作　　者：李宇飞 张春涛[1,2] 姜会梨 王瑜 赵冰骢 孙阳 高兴州 李想 李亚欢 冯世醒 图娅[1,2] LI Yu-fei;ZHANG Chun-tao;JIANG Hui-li;WANG Yu;ZHAO Bing-cong;SUN Yang;GAO Xing-zhou;LI Xiang;LI Ya-huan;FENG Shi-xing;TU Ya(School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China;Research Center of Mental and Neurological Disorders,School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China;Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]北京中医药大学针灸推拿学院精神神经疾患研究中心,北京100029 [3]中国中医科学院针灸研究所,北京100700

出　　处：《中华中医药杂志》2020年第4期1700-1704,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81373729,No.81574070)。

摘　　要：目的:观察针刺对慢性束缚应激模型(CRS)大鼠脑脊液载脂蛋白(APO)-A1、APO-D、视黄醇结合蛋白(RB P)的影响,探究针刺抗抑郁干预机制。方法:将32只雄性SD大鼠按随机数字表分为正常组、模型组、针刺组和氟西汀组。采用慢性束缚应激结合孤养方法建立模型,针刺组在CRS前1h针刺“百会”“印堂”“三阴交”穴,氟西汀组在CRS前1h采用氟西汀灌胃。采用旷场实验、体质量、糖水偏好实验观察大鼠行为学变化;采用酶联免疫吸附法检测脑脊液APO-A1、APO-D、RBP的表达。结果:与正常组比较,模型组大鼠体质量、水平穿越格数、竖立次数、糖水消耗量和糖水偏好指数均显著降低(P<0.05);与模型组比较,针刺组大鼠体质量、水平穿越格数和竖立次数、糖水消耗量、糖水偏好指数均显著增加(P<0.05)。ELISA结果显示,与正常组比较,模型组大鼠APO-A1、APO-D和RBP表达显著降低(P<0.05);与模型组比较,针刺组和氟西汀组大鼠APO-A1、APO-D和RBP表达显著升高(P<0.05)。结论:针刺干预通过逆转CRS诱导的模型大鼠脑脊液APO-A1、APO-D和RBP表达降低调节脑微环境,这可能是针刺抗抑郁效应的重要途径之一。Objective:To observe the effects of acupuncture on the apolipoprotein-A1(APO-A1),apolipoprotein-A1(APO-D)and retinol-binding protein(RBP)in the cerebrospinal fluid of chronic restrained-stress model rats.Methods:Thirty two male SD rats were randomly divided into control group,model group,acupuncture group and fluoxetine group.The animal model was established by using the combination of chronic restraint stress and solidarity.Rats in acupuncture group were acupunctured at Baihui(GV20),Yintang(GV29)and Sanyinjiao(SP6)point before CRS modeling.Rats in fluoxetine group were treated with fluoxetine gavage before modeling.Using open-field test,body weight and sucrose preference test to evaluate behavior change of depression model rats.Enzyme-linked immune sorbent assay(ELISA)was used to detect expression of APO-A1,APO-D and RBP in the cerebrospinal fluid.Results:Compared with control group,the number of the crossing and rearing,sucrose consumption,sucrose preference index and the body weight in model group showed a significant decrease(P<0.05).Compared with the model group,crossing and rearing,sucrose consumption,sucrose preference index and the body weight in the acupuncture group showed a significant increase(P<0.05).In the ELISA test,the model group showed a significant decrease of the expression of the APO-A1,APO-D and RBP compared with control group(P<0.05).Compared with model group,the expression of the APO-A1,APO-D and RBP in acupuncture and fluoxetine group showed a significant up regulation(P<0.05).Conclusion:Acupuncture intervention can regulate the brain microenvironment by reverse the down regulation of the expression of APO-A1,APO-D and RBP in the cerebrospinal fluid of the CRS rats,which may be an important way of the effect of antidepression by acupuncture.

关 键 词：针刺 脑脊液 慢性束缚应激 载脂蛋白 视黄醇结合蛋白 

分 类 号：R245[医药卫生—针灸推拿学]