猕猴桃褪绿环斑相关病毒在陕西省的分布、分子变异及其2个分离物的基因组序列  

Distribution,molecular characterization of Actinidia chlorotic ringspot-associated virus(AcCRaV)from Actinidia in Shaanxi and complete genome sequences of two isolates

作　　者：赵磊[1] 吴宽[1,2] 顾沛雯 曹孟籍[4] 王乔春 吴云锋 ZHAO Lei;WU Kuan;GU Peiwen;CAO Mengji;WANG Qiaochun;WU Yunfeng(State Key Laboratory of Crop Stress Biology for Arid Areas,Key Laboratory of Crop Pest Integrated Pest Management on Crop in Northwestern Loess Plateau,Ministry of Agriculture,College of Plant Protection,NorthwestA&F University,Yangling 712100,Shaanxi Province,China;Yangling Vocational&Technical College,Yangling 712100,Shaanxi Province,China;College of Agriculture,Ningxia University,Yinchuan 750002,Ningxia Hui Autonomous Region,China;Citrus Research Institute,Southwest University,Chongqing 400712,China;College of Horticulture,Northwest A&F University,Yangling 712100,Shaanxi Province,China)

机构地区：[1]西北农林科技大学植物保护学院,旱区作物逆境生物学国家重点实验室,农业部西北黄土高原作物有害生物综合治理重点实验室,陕西杨凌712100 [2]杨凌职业技术学院,陕西杨凌712100 [3]宁夏大学农学院,银川750002 [4]西南大学柑橘研究所,重庆400712 [5]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《植物保护学报》2020年第2期365-371,共7页Journal of Plant Protection

基　　金：宁夏回族自治区重点研发计划(2019BBF02013);陕西省重点研发计划(2018NY-101,2018ZDXM-NY-058)。

摘　　要：为明确我国陕西省猕猴桃主产区的徐香、海沃德、华优和秦美4个猕猴桃品种上的猕猴桃褪绿环斑相关病毒(Actinidia chlorotic ringspot-associated virus,AcCRaV)的分布情况,对采集自该省4个地区的493份样品进行AcCRaV检测,基于cp基因序列对获得的AcCRaV分离物进行分子变异分析,并采用高通量测序技术对其中2份样品进行转录组测序。结果显示:AcCRaV在陕西省猕猴桃上分布广泛,且检出率较高,其中周至县秦美猕猴桃上AcCRaV的检出率最高,为45.0%,在杨凌区秦美猕猴桃上AcCRaV的检出率最低,为10.0%。测定的23个AcCRaV分离物cp基因序列全长均为945 nt。系统发育树显示AcCRaV分离物共分成2个组,存在较大的分子变异。AcCRaV的cp基因分子变异与猕猴桃品种有一定关系,而与地理位置相关性不明显。获得了2个AcCRaV分离物ZZ1和ZZ2的基因组序列,RNA1长度分别为7049 nt和7274 nt,RNA2长度均为2266 nt,RNA3长度分别为1691 nt和1696 nt,RNA4长度分别为1736 nt和1683 nt,RNA5长度分别为1460 nt和1497 nt。分离物ZZ1和ZZ2的基因组序列与GenBank中唯一报道的我国湖北省AcCRaV分离物HN-6基因组序列比对中,分离物ZZ2与HN-6的RNA4同源率最高,为96.0%,分离物ZZ2与HN-6的RNA1同源率最低,为87.8%。表明AcCRaV在我国陕西省猕猴桃主产区分布较广泛,且其分子变异与猕猴桃品种有一定关系。To investigate the incidence and distribution of Actinidia chlorotic ringspot-associated virus(AcCRaV)in the four major kiwifruit cultivars Xuxiang,Hayward,Huayou and Qinmei grown in Shaanxi Province of China,a total of 493 samples from the four regions of Shaanxi were collected for AcCRaV detection.Their molecular variations were analyzed based on cp gene sequences,and transcriptome sequencing was also conducted in two isolates.The results showed that AcCRaV was widespread in kiwifruit in Shaanxi,with the highest detection rate in the cultivar Qinmei from Zhouzhi County(45.0%)and the lowest in the cultivar Qinmei from Yangling(10.0%).The full-length cp gene sequences were obtained from 23 AcCRaV isolates.The phylogenetic tree showed that these isolates were divided into two groups,with large molecular variations.The molecular variation of AcCRaV cp gene was related to different kiwifruit cultivars,but its correlation with different geographical regions was not significant.In this study,genome sequences of two AcCRaV isolates(ZZ1 and ZZ2)were obtained,with sequence lengths of 7049 nt and 7274 nt for RNA1,both 2266 nt for RNA2,1691 nt and 1696 nt for RNA3,1736 nt and 1683 nt for RNA4,and 1460 nt and 1497 nt for RNA5,respectively.The genomes of the two isolates were compared with that of the Hubei isolate HN-6,which was the only entry from China in GenBank.Among the five RNA strands,the highest nucleotide identity(96.0%)was observed between ZZ2 and HN-6 for RNA4,while the lowest nucleotide identity(87.8%)was observed between ZZ2 and HN-6 for RNA1.It indicated that AcCRaV was widely distributed in the four kiwifruit cultivars in Shaanxi,and its molecular variation was related to different kiwifruit cultivars.

关 键 词：猕猴桃 猕猴桃褪绿环斑相关病毒 基因组 分子变异 陕西 

分 类 号：S432.41[农业科学—植物病理学]