硫辛酸对镉暴露引起的蛋鸡肝脏脂肪沉积、氧化应激、元素稳态失衡的保护效应  

Protective effects of lipoic acid on liver fat deposition, oxidative stress and element homeostasis imbalance in layer hens induced by cadmium exposure

作　　者：陈大伟[1] 马丽娜[1] 刘茵茵[1] 王倩倩 蒲俊华[1] 陆俊贤[1] 葛庆联[1] 唐修君[1] 高玉时[1] CHEN Dawei;MA Lina;LIU Yinyin;WANG Qianqian;PU Junhua;LU Junxian;GE Qinglian;TANG Xiujun;GAO Yushi(Jiangsu institute of poultry science,Laboratory of Quality and Safety Risk Assessment for Poultry Products Ministry of agriculture(Yangzhou)Yangzhou 225125,China;Yangzhou Animal Husbandry and Veterinary Station,Yangzhou 225009,China)

机构地区：[1]江苏省家禽科学研究所农业农村部禽类产品质量安全风险评估实验室(扬州),江苏扬州225125 [2]扬州市畜牧兽医站,江苏扬州225009

出　　处：《畜牧与兽医》2020年第4期45-50,共6页Animal Husbandry & Veterinary Medicine

基　　金：十三五国家重点研发计划项目(2016YFD0501208);江苏现代农业(肉鸡)产业技术体系(JATS[2019]382);扬州市社会发展项目(YZ2018073);省属公益类科研院所自主科研经费(BM2018026)。

摘　　要：本研究旨在探讨α、硫辛酸(α-LA)对镉(Cd)暴露蛋鸡肝脏脂肪沉积、氧化应激和元素稳态的保护效应。将360只42周龄京粉2号商品代蛋鸡随机分为4组,分别为对照组、α-硫辛酸组、镉组和镉与α-硫辛酸共处理组,镉组在饲料中添加CdCl2100 mg/kg,共处理组在镉暴露的基础上同时用添加硫辛酸400 mg/kg,8周后测定血清生化指标、肝脏脂质过氧化指标、微量元素含量,观察肝脏解剖学及病理学变化。结果表明,α-LA能显著恢复镉暴露引起的蛋鸡肝脏超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和还原型谷胱甘肽(GSH)含量;降低镉暴露引起的丙二醛(MDA)含量的升高、血清谷草转氨酶(AST)、谷丙转氨酶(ALT)活性升高及甘油三酯(TG)和低密度脂蛋白(LDL)含量升高。α-LA降低了镉暴露引起的蛋鸡肝脏脂肪沉积,减少了肝脏镉蓄积和铁的积累。以上结果显示,α-LA对镉暴露引起的蛋鸡肝脏脂肪变性及损伤具有一定的保护效应。The purpose of this study was to investigate the protective effects of α-Lipoic acid on liver fat deposits, oxidative stress and element homeostasis. 360 42-week-old Beijing Pink 2 laying hens were divided into 4 groups randomly: the control group, the alpha LA-treated group, the Cd-treated group, and the Cd and alpha LA co-treated group. The Cd-treated group was exposed to CdCl2(100 mg/kg) in feed, and and the Cd and alpha LA co-treated group to alpha LA(400 mg/kg) besides CdCl2. Eight weeks later, the biochemical indexes in the serum of the subjects, the peroxidation index in their liver lipid, the contents of trace elements in their liver were measured, and changes in liver anatomy and pathology of the hens were also observed. The results showed that alpha LA effectively restored the SOD and GSH-Px activities and the GSH content in the liver of the laying hen exposed to cadmium, and the acid reduced the increase in MDA caused by cadmium exposure. The activity of AST and ALT in the serum of the hens increased, and the contents of TG and LDL increased, too. Alpha LA reduced the accumulation of cadmium and iron in the liver of the laying hens. These results indicated that alpha LA had a protective effect on steatosis in and injury of the liver of cadmiumexposed laying hens.

关 键 词：镉 硫辛酸 蛋鸡 脂肪沉积 

分 类 号：S856.9[农业科学—临床兽医学]