正交试验优选猫爪草纳米乳喷雾剂的提取工艺  

Optimization of extraction process for Ranunculi Ternati Radix nanoemulsion spray based on orthogonal test

作　　者：曾春姣 陈玲珑[2] 李跃辉[3] ZENG Chunjiao;CHEN Linglong;LI Yuehui(Xiangtan Medicine&Health Vocational College,Xiangtan 411000,Hunan,China;The Affiliated Hospital of Hunan Academy of Chinese Medicine,Changsha 410006,Hunan,China;Hunan Academy of Chinese Medicine,Changsha 410006,Hunan,China)

机构地区：[1]湘潭医卫职业技术学院,湖南湘潭411000 [2]湖南省中医药研究院附属医院,湖南长沙410006 [3]湖南省中医药研究院,湖南长沙410006

出　　处：《湖南中医杂志》2020年第4期150-152,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省教育厅科学研究项目(17C1568);湖南省湘潭市科技局指导计划项目(ZJ20171026)。

摘　　要：目的:正交试验优选猫爪草纳米乳喷雾剂的最佳提取工艺。方法:以总黄酮提取量和浸膏得率的综合评分为评价指标,在单因素试验基础上,采用L9(34)正交试验考察猫爪草醇提工艺的溶剂体积、乙醇浓度、提取时间及提取温度对提取工艺的影响。结果:最佳提取工艺为以75%乙醇为提取溶剂,提取温度60℃,第1次8倍量1.5 h,第2次7倍量1 h,共提取2次。结论:在单因素试验基础上,通过正交试验优选的提取工艺经验证稳定、简便易行、预测性好,为猫爪草纳米乳喷雾剂的进一步研发奠定了基础。Objective:To optimize the extraction process for Ranunculi Ternati Radix nanoemulsion spray based on orthogonal test.Methods:With the extraction yield of total flavonoids and the extract yield as assessment indices,based on a univariate experiment,the L9(34)orthogonal test was used to evaluate the influence of solvent volume,alcohol concentration,extraction duration,and extraction temperature on the alcohol extraction process for Ranunculi Ternati Radix.Results:The optimal extraction process was as follows:75%alcohol as the extraction solvent,an extraction temperature of 60℃,and extraction twice,with 8 times amount for 1.5 hours followed by 7 times amount for 1 hour.Conclusion:The extraction process optimized based on univariate experiment and orthogonal test is stable,simple,and convenient with good predictability,which lays a foundation for further research and development of Ranunculi Ternati Radix nanoemulsion spray.

关 键 词：猫爪草纳米乳喷雾剂 总黄酮 浸膏得率 正交试验 

分 类 号：R284.2[医药卫生—中药学]