具有抗稻瘟病菌活性的ⅡW1菌株的分离鉴定及其接合转移方法的建立  

Isolation and identification of the ⅡW1 strain with antifungal activity to Pyricularia oryzae and its conjugation transfer

作　　者：李晓华[1] 冯喆 黄粤 马婷婷 姚家成 夏珍珍 LI Xiaohua;FENG Zhe;HUANG Yue;MA Tingting;YAO Jiacheng;XIA Zhenzhen(Hubei Provincial Engineering and Technology Research Center for Resources and Utilization of Microbiology/Key Laboratory for Biotechnology of the State Ethnic Affairs Commission,College of Life Sciences,South-Central University for Nationalities,Wuhan 430074,China)

机构地区：[1]中南民族大学生命科学学院,微生物资源与利用湖北省工程技术研究中心/生物技术国家民委重点实验室,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2020年第3期240-244,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(31070087);中央高校基本科研业务费专项资金资助项目(CZY19018)。

摘　　要：从湖北省神农架地区土壤中筛选出一株具有抗稻瘟病菌活性的ⅡW1菌株,采用菌块法测定了ⅡW1菌株对稻瘟病菌的抑菌活性,通过形态结构、生理生化和16S rDNA基因序列同源性比对对ⅡW1菌株进行鉴定,同时对链霉菌ⅡW1菌株attB序列进行克隆,并建立接合转移的方法.结果表明:ⅡW1菌株属假灰色链霉菌,链霉菌ⅡW1菌株对稻瘟病NO-1菌株和168菌株的抑菌圈直径分别为29.7 mm和27.4 mm;链霉菌ⅡW1菌株attB序列具有位点特异性重组所必须的核心位点5′-TT-3′.含ΦC31attP位点的整合型质粒pSET152通过两亲接合转移导入链霉菌ⅡW1菌株.TheⅡW1 strain resistant to Pyricularia oryzae was isolated from planting soils in Shennongjia District of Hubei Province.The antibacterial activity against Pyricularia oryzae of theⅡW1 strain was determined by the method of bacteria block.TheⅡW1 strain was identified by morphological structure,physiological and biochemical,and 16S rDNA gene sequence homology alignment.Meanwhile,the method of conjugation transfer for theⅡW1 strain was established by cloning of its attB sequence.The results showed that theⅡW1 strain was identified as Streptomyces pseudogriseolus and the inhibition zone diameter of the Streptomyces pseudogriseolusⅡW1 strain against Pyricularia oryzae NO-1 and 168 was 29.7 mm and 27.4 mm,respectively.The attB sequence of the Streptomyces pseudogriseolusⅡW1 strain had 5′-TT-3′necessary core site for site-specific recombination.The integrated plasmid pSET152 containing the ΦC31attP site was transferred to Streptomyces pseudogriseolusⅡW1 strain by conjugation transfer.

关 键 词：假灰色链霉菌ⅡW1菌株 稻瘟病菌 attB位点 接合转移 

分 类 号：Q939[生物学—微生物学] S182[农业科学—农业基础科学]