细胞色素P45055B1的直接电化学及对一氧化氮的电催化检测  

Direct electrochemistry of cytochrome P45055B1 and electrocatalytic detection of nitric oxide

作　　者：姜楠 吴云华[1] JIANG Nan;WU Yunhua(College of Life Sciences,South-Central University for Nationalities,Wuhan 430074,China)

机构地区：[1]中南民族大学生命科学学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2020年第3期245-249,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(31670372);中央高校基本科研业务费专项资金资助项目(CZZ18004)。

摘　　要：采用戊二醛(GA)交联法将细胞色素P45055B1(CYP55B1)固定在热解石墨电极(PGE)表面,牛血清白蛋白(BSA)封闭多余活性位点,构建了一种新型一氧化氮(NO)生物传感器.并考察了电化学生物传感器的选择性与稳定性.结果表明:CYP55B1实现了直接电子传递,氧化还原峰电位位于-0.355 V和-0.385 V,CYP55B1对NO具有电催化活性,其催化还原峰位于-0.85 V.NO的检测线性范围为14.4~108μmol·L^-1,检测限为10.47μmol·L^-1,且该生物传感器具有良好的稳定性及选择性.Cytochrome P45055B1(CYP55B1)was immobilized on the surface of pyrolytic graphite electrode(PGE)by glutaraldehyde(GA)crosslinking,and bovine serum albumin(BSA)was applied to block the excess active sites.A new nitric oxide biosensor was thus developed.The selectivity and the stability of the electrochemical biosensor were investigated.The results showed that direct electron transfer of CYP55B1 was realized and its oxidative and reductive peak potential was-0.355 V and-0.385 V,respectively.CYP55B1 demonstrated good electrocatalytic activity to nitric oxide,and the catalytic reductive peak potential for nitric oxide was-0.85 V.The linear range of nitric oxide detection was from 14.4 to 108μmol·L^-1 and the detection limit was 10.47μmol·L^-1.The biosensor also demonstrated good stability and selectivity.

关 键 词：细胞色素P45055B1 一氧化氮 热解石墨电极 电化学传感器 一氧化氮还原酶 

分 类 号：Q599[生物学—生物化学]