植物内生真菌来源的倍半萜对LPS诱导细胞炎症反应的抑制作用  

Inhibition of LPS-induced cell inflammatory response by sesquiterpenoid derived from endophytic fungi

作　　者：黄蓉 王滟 张宪栋 杨会祥 刘吉开[1] HUANG Rong;WANG Yan;ZHANG Xiandong;YANG Huixiang;LIU Jikai(National Demonstration Center for Experimental Ethnopharmacology Education,School of Pharmaceutical Sciences,South-Central University for Nationalities,Wuhan 430074,China)

机构地区：[1]中南民族大学药学院,民族药学国家级实验教学示范中心,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2020年第3期255-258,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(GZQ15015);中央高校基本科研业务费专项资金资助项目(CZQ19013)。

摘　　要：目的:探究马铃薯内生真菌次生代谢产物中新型倍半萜类化合物TA的抗炎作用.方法:脂多糖(LPS)刺激RAW264.7细胞建立体外炎症模型,通过测定细胞存活率、细胞NO的产生量、细胞内活性氧(ROS)水平,细胞培养上清液中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的释放量、一氧化氮合成酶(iNOS),环氧合酶(COX-2)蛋白表达量,从而评价化合物TA的抗炎活性.结果:化合物TA对细胞无毒性,可剂量依赖性地抑制LPS刺激产生的NO,降低ROS水平,减少细胞炎症因子TNF-α和IL-1β的产生及下调iNOS,COX-2蛋白的表达量.结论:马铃薯内生真菌次生代谢产物中新型倍半萜类化合物TA具有显著的抗炎活性.Objective:To investigate the anti-inflammatory effect of a new sesquiterpene compound TA in the secondary metabolites of potato endophytic fungi.Methods:Lipopolysaccharide(LPS)stimulated RAW264.7 cells to establish an in vitro inflammation model.Cell viability,nitrite levels representing NO production,intracellular reactive oxygen species(ROS)levels,the release of inflammatory cytokines into the supernatant such as tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)were measured.Moreover,Western-blotting assays were used to detect the expression of induced-nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2)proteins to evaluate the anti-inflammatory activity of compound TA.Results:Compound TA was non-toxic to cells,and could inhibit NO produced,reduce ROS level,decrease the production of inflammatory cytokines and down-regulate the expression of iNOS and COX-2 proteins in a dose-dependent manner.Conclusion:TA,a novel sesquiterpene compound in the secondary metabolites of potato endophytic fungi,has significant anti-inflammatory activity.

关 键 词：倍半萜 抗炎 植物内生真菌 RAW264.7细胞 

分 类 号：Q493[生物学—生理学]