诱导人胚胎干细胞向子宫内膜样细胞分化的初步研究  

Differentiation of Human Embryonic Stem Cell to Endometrium-Like Cell:A Pilot Study

作　　者：郭杉 袁涛 刘英[1] GUO Shan;YUAN Tao;LIU Ying(Department of Reproductive Medicine,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing 100026,China)

机构地区：[1]首都医科大学附属北京妇产医院生殖医学科,北京100026

出　　处：《国际生殖健康／计划生育杂志》2020年第3期190-194,共5页Journal of International Reproductive Health/Family Planning

基　　金：国家自然科学基金(81471520);北京市自然科学基金(7182054);北京市科技新星计划交叉学科合作课题(Z191100001119015)。

摘　　要：目的:探讨无饲养层培养的人胚胎干细胞诱导分化为子宫内膜样细胞的方法。方法:将无饲养层培养的人胚胎干细胞分别通过共培养诱导法和细胞因子诱导法向子宫内膜样细胞诱导,流式细胞术检测分化的细胞中子宫内膜上皮细胞标志物细胞角蛋白18和间质细胞标志物波形蛋白的表达情况。结果:共培养诱导法和细胞因子诱导法在分化第7天均出现长梭状细胞样改变。分化第8天通过流式细胞术检测发现细胞波形蛋白表达均为阴性,部分细胞角蛋白18表达阳性,共培养诱导法分化率高于细胞因子诱导法[(55.63±10.29)%vs.(13.90±0.26)%,P<0.05]。结论:共培养诱导法和细胞因子诱导法均可使人胚胎干细胞分化为子宫内膜上皮样细胞,但前者的分化效率更高。Objective:To explore the differentiation methods of the feeder-free cultured human embryonic stem cell(hESCs)to human endometrium-like cell.Methods:The hESCs were differentiated in vitro using the cocultured method and the cytokine-induced method.Flow cytometry was applied to detect the differentiation efficiency of hESCs by human endometrium epithelial marker cytokeratin-18(CK-18)and human endometrium mesenchymal marker vimentin(VIM).Results:The hESCs cultured with the two methods represented the elongated and spindle shape on day7.Flow cytometry analysis on day 8 showed that the differentiated cells were all negative for vimentin and partially positive for CK-18.Compared to the cytokine-induced method,the co-cultured method obtained more endometrium epithelial-like cells[(55.63±10.29)%vs.(13.9±0.26)%,P<0.05].Conclusions:hESCs were successfully induced to differentiate to endometrium epithelial-like cells by both the co-cultured method and the cytokine-induced method.Compared to the cytokine-induced method,the co-cultured method can obtain endometrium epithelial-like cells more efficiently.

关 键 词：人胚胎干细胞 诱导多能干细胞 细胞分化 子宫内膜 上皮细胞 细胞培养技术 无饲养层 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]