左归饮对肾阴虚衰老大鼠氧化应激反应的影响  

Effect of Zuogui Decoction on Oxidative Stress Response in Senescent Rats with Kidney Yin Deficiency

作　　者：何帆 江婷 洪世忠 陈卫东 刘李 HE Fan;JIANG Ting;HONG Shi-zhong;CHEN Wei-dong;LIU Li(School of Pharmacy,Anhui University of Chinese Medicine,Anhui Hefei 230012,China;Anhui Academy of Chinese Medicine,Anhui Hefei 230012,China;Institute of Drug Metabolism,Anhui University of Chinese Medicine,Anhui Hefei 230012,China;School of Medical Economics Management,Anhui University of Chinese Medicine,Anhui Hefei 230012,China;College of Pharmacy,China Pharmaceutical University,Jiangsu Nanjing 210009,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]安徽省中医药科学院,安徽合肥230012 [3]安徽中医药大学药物代谢研究所,安徽合肥230012 [4]安徽中医药大学医药经济管理学院,安徽合肥230012 [5]中国药科大学药学院,江苏南京210009

出　　处：《安徽中医药大学学报》2020年第3期59-62,共4页Journal of Anhui University of Chinese Medicine

基　　金：国家自然科学基金项目(81773988);安徽省自然科学基金项目(KJ2017A29T)。

摘　　要：目的探讨左归饮水提液延缓肾阴虚大鼠衰老的机制。方法将SD大鼠随机分为5组,分别为正常组,模型组,左归饮高、中、低剂量组,模型组每日灌胃给予甲状腺素混悬液150mg/kg,左归饮高、中、低剂量组分别灌胃左归饮水提液31、20.67、13.78g/kg,连续7d。检测大鼠血清超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、肌酐(creatinine,Cr)、血尿素氮(blood urea nitrogen,BUN)、环磷酸腺苷(cyclic adenosine monophosphate,cAMP)、环磷酸鸟苷(cyclic guanosine monophosphate,cGMP)水平及cAMP/cGMP比值。结果与模型组比较,左归饮高、中、低剂量组大鼠血清cAMP水平,cAMP/cGMP均显著降低(P<0.05),而cGMP水平无显著变化;与模型组比较,左归饮中、高剂量组大鼠血清SOD、CAT、GSH-Px水平均显著升高(P<0.05),MDA水平显著下降(P<0.05);与模型组比较,左归饮低、中、高剂量组大鼠血清Cr和BUN水平均显著降低(P<0.05)。结论左归饮通过抗氧化作用延缓肾阴虚大鼠衰老。Objective To investigate the mechanism of water extract of Zuogui Decoction in delaying senescence of rats with kidney Yin deficiency.Methods Sprague-Dawley rats were randomly divided into normal group,model group,and high-,middle-,and low-dose Zuogui Decoction groups.The rats in the model group were given thyroxine suspension 150 mg/kg every day by gavage,and those in the high-,middle-,and low-dose Zuogui Decoction groups were given water extract of Zuogui Decoction at a dose of31,20.67,and 13.78 g/(kg·d),respectively,by gavage.The course of treatment was 7 consecutive days.The levels of superoxide dismutase(SOD),malondialdehyde(MDA),catalase(CAT),glutathione peroxidase(GSH-Px),creatinine(Cr),blood urea nitrogen(BUN),cyclic adenosine monophosphate(cAMP),and cyclic guanosine monophosphate(cGMP)were measured,and cAMP/cGMP ratio was calculated.Results Compared with the model group,the high-,middle-,and low-dose Zuogui Decoction groups had a significant reduction in the serum level of cAMP(P<0.05);the three Zuogui Decoction groups had no significant change in the level of cGMP,with a significant reduction in cAMP/cGMP ratio(P<0.05).Compared with the model group,the middle-and high-dose Zuogui Decoction groups had significant increases in the serum levels of SOD,CAT,and GSH-Px(P<0.05)and a significant reduction in MDA level(P<0.05).Compared with the model group,the low-,middle-,and high-dose Zuogui Decoction groups had significant reductions in the serum levels of Cr and BUN(P<0.05).Conclusion Zuogui Decoction can delay senescence of rats with kidney Yin deficiency by exerting an antioxidant effect.

关 键 词：左归饮 肾阴虚 抗氧化 

分 类 号：R289.5[医药卫生—方剂学]