电针不同穴位对肠易激综合征大鼠精神行为及NMDA受体表达的影响研究  

Effect of Electroacupuncture at Different Acupoints on the Mental Behaviors and NMDA Receptors Expressions of IBS Rats

作　　者：覃颖 王一凡 郭孟玮[1] 蓝莹[1] 王珊[1] 吉毛先 朱文莲[1] 任晓暄[1] Qin Ying;Wang Yifan;Guo Mengwei;Lan Ying;Wang Shan;Ji Maoxian;Zhu Wenlian;Ren Xiaoxuan(Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029

出　　处：《中国中医急症》2020年第5期773-775,796,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81473776);北京中医药大学校级课题(2030072120016)。

摘　　要：目的观察电针不同穴位对肠易激综合征(IBS)大鼠身心症状及前扣带回皮层(ACC)中NMDA受体表达的影响,探讨不同穴位对IBS的疗效差异及其相关机制。方法新生Wistar大鼠分为空白对照组、模型对照组、印堂组和大椎组,每组10只。除空白对照组外,均制备IBS模型。2月龄时,两电针组给予电针刺激,每次20 min,隔日1次,共5次。治疗结束后,采用腹部回撤反射(AWR)评估肠道敏感性;旷场试验(OFT)评估大鼠的精神行为;实时荧光定量PCR检测ACC中NMDA受体(NR1、NR2A、NR2B)的表达。结果与空白对照组比较,模型对照组的潜伏期明显缩短、收缩波个数明显增多(P<0.01),水平和垂直运动次数明显减少(P<0.01),NR1、NR2A、NR2B的表达均增加(P<0.05);与模型对照组比较,印堂组的潜伏期明显延长、收缩波个数减少(P<0.01),水平和垂直运动次数均明显增多(P<0.01),NR1、NR2A、NR2B的表达降低(P<0.05),大椎组的潜伏期延长(P<0.05),NR1、NR2B的表达降低(P<0.05);与印堂组比较,大椎组的收缩波个数明显增多(P<0.01),水平运动次数明显减少(P<0.01)。结论电针"印堂""大椎"穴均可有效改善IBS模型大鼠的抑郁样精神状态,但电针"印堂"穴的效应更佳,且二者对NR2A的阳性表达亦存在差异。Objective:To observe the effect of electroacupuncture(EA)at different acupoints on the physical and mental symptoms of IBS rats and the expression of NMDA receptor in ACC,and to explore the therapeutic effect of different acupoints on IBS and its related mechanism. Methods:40 newborn Wistar rats were randomly divided into the blank group,the model group,Yintang(GV 29)group and Dazhui(GV 14)group,10 rats in each.IBS model was built except the blank group. 2-month-old rats in GV 29 group and GV 14 group were given EA intervention once every other day for 5 times,20 minutes for each time. After the treatment,abdominal withdrawal reflex(AWR)was used to evaluate the intestinal sensitivities. Open field test(OFT)was used to evaluate the emotional behavior of rats. The expression of NMDA receptors(NR1,NR2A,NR2B)in ACC was detected with RTPCR. Results:Compared with the black group,the latency of model group was significantly shortened and the number of contraction waves was significantly increased(P < 0.01),while the numbers of vertical and horizontal movements were also significantly reduced(P < 0.01);NR1,NR2A and NR2B in ACC of rats increased in the model group(P < 0.05). Compared with the model group,the latency was significantly prolonged and the number of contraction wave was significantly decreased(P < 0.01);the numbers of vertical and horizontal movements were significantly increased(P < 0.01),and NR1,NR2A and NR2B in ACC also reduced in GV 29 group(P < 0.05),while the latency prolonged and NR1 and NR2B in ACC reduced in GV 14 group(P < 0.05). Compared with GV 29 group,the number of contraction wave was significantly increased and the horizontal movements were significantly decreased in GV 14 group(P < 0.01). Conclusion:EA at GV 29 or GV 14 can effectively improve the depressive mood in IBS rat. GV 29 has a better effect,and there are differences in the expression of NR2A in ACC.

关 键 词：肠易激综合征 电针 印堂 大椎 肠道敏感性 N-甲基-D-天冬氨酸 大鼠 

分 类 号：R285.5[医药卫生—中药学]