丹参酮ⅡA对大鼠心肌缺血再灌注损伤中HMGB1、IL-1β表达及SOD水平的影响  

Effects of TanshinoneⅡA on the Expressions of HMGB1,IL-1βand SOD in Myocardial Ischemia-reperfusion Injury in Rats

作　　者：丁华胜 王永剑 黄忠毅 Ding Huasheng;Wang Yongjian;Huang Zhongyi(Shenzhen Hospital of Southern Medical University,Guangdong,Shenzhen 518101,China)

机构地区：[1]南方医科大学深圳医院,广东深圳518101

出　　处：《中国中医急症》2020年第5期776-778,801,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金青年项目(81401304)。

摘　　要：目的探讨丹参酮ⅡA对大鼠心肌缺血再灌注损伤中HMGB1、IL-1β表达及SOD的影响。方法将清洁级成年雄性SD大鼠随机分为假手术组、模型组和丹参酮ⅡA高、低剂量组,在缺血前15 min分别向各组大鼠注射生理盐水或丹参酮ⅡA磺酸钠注射液。通过结扎左冠脉前降支40 min,再灌注2 h建立大鼠心肌缺血再灌注损伤模型。采用比色法测定SOD的含量,ELISA法测定血清白介素-1β(IL-1β)水平。取心脏,TTC染色法测定心肌梗死面积,免疫组化检测HMGB1蛋白表达。结果与模型组比较,丹参酮ⅡA低剂量组和丹参酮ⅡA高剂量组SOD活性升高,血清IL-1β表达减少,心肌组织HMGB1蛋白水平明显降低,其中丹参酮ⅡA高剂量组差异更显著(P<0.05或P<0.01)。结论丹参酮ⅡA预处理可通过减少HMGB1与增强SOD活性,降低心肌梗死面积,从而实现对心肌缺血再灌注损伤的保护。Objective:To investigate the effect of TanshinoneⅡA on the expressions of HMGB1,IL-1βand SOD in rats with myocardial ischemia-reperfusion injury.Methods:Clean adult male SD rats were randomly divided into 4 groups with 13 rats in each:sham operation group,the model group and TanshinoneⅡA high and low dose groups.The groups were injected with 4 m L of normal saline,4 m L of normal saline and sodium TanshinoneⅡA sulfonate respectively 15 minutes before ischemia.The model of myocardial ischemia-reperfusion injury was established by ligating the anterior descending branch of left coronary artery for 40 minutes and reperfusion for 2 hours.The content of SOD was determined by colorimetry,and the level of IL-1βwas determined by ELISA.The area of myocardial infarction was measured by TTC staining and the expression of HMGB1 protein was detected by immunohistochemistry.Results:Compared with the model group,the activity of SOD,the expression of IL-1βin serum and the level of HMGB1 protein in myocardial tissue increased in low dose and high dose TanshinoneⅡA groups,and the difference was more significant in high dose group(P<0.05 or P<0.01).Conclusion:TanshinoneⅡA pretreatment can reduce HMGB1 and enhance SOD activity and reduce myocardial infarction area,so as to achieve the protection of myocardial ischemia-reperfusion injury.

关 键 词：心肌缺血再灌注损伤 丹参酮ⅡA 高迁移率族蛋白B1 炎性反应 超氧化物歧化酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]