对照品替代法用于莪术油中3个倍半萜类成分的含量测定研究  

Studies on the Determination of Three Sesquiterpenoids in the Zedoary Turmeric Oil by Substitution of Reference Substance Method

作　　者：唐登峰 罗镭 马临科 陈碧莲 TANG Dengfeng;LUO Lei;MA Linke;CHEN Bilian(Zhejiang Institute for Food and Drug Control,NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Medicine(Traditional Chinese Patent Medicine),Hangzhou 310052,China)

机构地区：[1]浙江省食品药品检验研究院,国家药品监督管理局中成药质量评价重点实验室,杭州310052

出　　处：《中国现代应用药学》2020年第4期447-454,共8页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省科技厅分析测试科技计划项目(2016C37064)。

摘　　要：目的建立对照品替代法(substitution of reference substance method,SRSM)测定莪术油中3种倍半萜类成分的含量。方法采用C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-水为流动相,梯度洗脱,流速为1.0 mL·min^-1,检测波长为216 nm,柱温为30℃。以牻牛儿酮为参照物,计算呋喃二烯、莪术二酮的相对校正因子和相对保留时间,采用外标法和SRSM分别测定它们在莪术油中的含量。结果 3种成分在各自范围内线性关系良好(R2≥0.999 9),相对校正因子及相对保留时间稳定,SRSM测定结果与外标法测定结果无显著性差异。结论建立的SRSM操作简单、重复性好,能够替代外标法用于莪术油中3种倍半萜类成分的含量测定。OBJECTIVE To develop substitution of reference substance method(SRSM) for simultaneous determination of furanodiene, curdione and germacrone in the Zedoary Turmeric oil. METHODS The determination of furanodiene, curdione and germacrone were performed on a C18 analytical column(4.6 mm×250 mm, 5 μm) with acetonitrile-water as mobile phase in a gradient elution program at a flow rate of 1.0 mL·min-1. The column temperature was maintained at 30 ℃. The detection wavelength was 216 nm. Germacrone was used as the internal reference substance, RCFs and RRTs of furanodiene and curdione were calculated. The contenets of furanodiene and curdione were determined by both the external standard method(ESM) and SRSM. RESULTS Furanodiene, curdione and germacrone had a good linearity rangings respectively(R2≥0.999 9). RCFs and RRTs of furanodiene and curdione were stable. There was no significant difference between the results of SRSM and ESM. CONCLUSION The developed SRSM is simple and stable, which can determine the contents above mentioned in the Zedoary Turmeric oil instead of the ESM.

关 键 词：对照品替代法 倍半萜类成分 莪术油 

分 类 号：R284.1[医药卫生—中药学]