发酵虫草菌粉指纹图谱研究  

Fingerprint Study of Cultured Cordyceps Sinensis Powder

作　　者：杨悦 夏聪华 张菊芳 周屹峰[1] YANG Yue;XIA Conghua;ZHANG Jufang;ZHOU Yifeng(Hangzhou Zhongmei Huadong Pharmaceutical Co.,Ltd.,Hangzhou 310000,China;Huadong Medicine Co.,Ltd.,Hangzhou 310000,China)

机构地区：[1]杭州中美华东制药有限公司,杭州310000 [2]华东医药股份有限公司,杭州310000

出　　处：《中国现代应用药学》2020年第4期460-462,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的通过对发酵虫草菌粉指纹图谱的深入研究,为发酵虫草菌粉类产品的质量提高提供思路。方法液质联用确定发酵虫草菌粉类产品(百令胶囊)指纹图谱中6个主色谱峰的化学成分;采用中药色谱指纹图谱相似度评价系统,比较天然虫草和不同厂家发酵虫草菌粉指纹图谱相似度及不同干燥方式对指纹图谱的影响。结果百令胶囊指纹图谱中的6个主色谱峰成分为尿苷5-单磷酸、鸟苷酸、5’-腺嘌呤核苷酸、尿苷、鸟苷、腺苷。发酵虫草菌粉的指纹图谱与天然虫草均存在差异,其中百令胶囊的指纹图谱与天然虫草最接近。干燥方式对指纹图谱影响较大,采用沸腾干燥方式对应的指纹图谱主色谱峰的面积比最接近天然虫草晒干方式。结论指纹图谱可准确反映发酵虫草菌粉质量及质量控制方式的有效性。OBJECTIVE To provide strategies for quality improvement of cultured Cordyceps sinensis powder by detailed analysis on its HPLC fingerprint. METHODS Using LC-MS on cultured Cordyceps sinensis powder(Bailing capsule), six major chromatographic peaks in the fingerprint were identified. A similarity measurement based on Chinese traditional medicine fingerprinting system was used to compare similarities between cultured cordyceps and natural cordyceps, as well as the effect of drying methods on fingerprint profiles. RESULTS The 6 major peaks in the fingerprint of Bailing capsule had been identified as: uridine-5-monophosphate, guanylic acid, adenosine-5’-monophosphoric acid, uridine, guanosine and adenosine. The fingerprint of cultured Cordyceps sinensis powder differs from that of the natural cordyceps, while Bailing capsule had been shown to be most similar to natural cordyceps. The drying method also had a significant effect on the fingerprint. The product prepared by boil drying method produced the most similar fingerprint profile to the sun dried natural cordyceps. CONCLUSION The fingerprinting can accurately reflect the quality of cultured Cordyceps sinensis powder and the effectiveness of quality control.

关 键 词：发酵虫草菌粉 指纹图谱 液质联用 干燥方式 

分 类 号：R917.101[医药卫生—药物分析学]