检测大肠杆菌的免疫生物传感器研究进展  

Research progress on immunobiosensor for Escherichia coli detection

作　　者：王春皓 崔传金 谷学静 李静 陈宏硕 张学超[3] WANG Chunhao;CUI Chuanjin;GU Xuejing;Li Jing;CHEN Hongshuo;ZHANG Xuechao(School of Electrical Engineering,North China University of Science and Technology,Tangshan 063210;School of Engineering,Shanxi Agricultural University,Taigu 030801;Tongren University,Tongren 554300)

机构地区：[1]华北理工大学电气工程学院,唐山063210 [2]山西农业大学工学院,太谷030801 [3]贵州铜仁学院,铜仁554300

出　　处：《分析试验室》2020年第4期490-496,共7页Chinese Journal of Analysis Laboratory

基　　金：河北省教育厅项目(ZD2016070);河北省食品药品监督管理局项目(QN2015012);黔教合KY字[2018]342号和黔科合LH字[2017]7328资助。

摘　　要：大肠杆菌是人体内常见的食源性致病菌,对其检测极为重要。免疫生物传感器技术作为目前研究较热的一种技术,在大肠杆菌的检测方面有不错的进展。本文综述了电化学、光学、压电等类型免疫传感器近十年的研究进展,从检测原理、抗体固定方式、修饰材料、检测能力等方面进行了综述,并对各自的优劣势进行了讨论,对免疫传感器的发展趋势进行了总结与展望。Detection of Escherichia coli is extremely important,mainly because it is a common foodborne pathogen in human body.Immunobiosensor technology as one of the hottest research technologies at present has made good progress in the detection of Escherichia coli.In this paper,the progress of electrochemical,optical and piezoelectric immunobiosensors in recent ten years are reviewed.The advantages and disadvantages of different sensors are discussed from detection principle,antibody immobilization method,modification materials and detection ability.Finally,the immunobiosensor’s development trend is summarized and prospected.

关 键 词：大肠杆菌 抗体 电化学免疫生物传感器 压电免疫生物传感器 光学免疫生物传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]