尖孢镰刀菌全基因组测序及其致病相关基因分析  

Whole Genome Sequencing of Fusarium oxysporum and Analysis of Its Pathogenic Related Genes

作　　者：文增叶 李定华 代梦瑶 杨野[1] 杨启良[2] 崔秀明[1] 年洪娟[1] Wen Zengye;Li Dinghua;Dai Mengyao;Yang Ye;Yang Qiliang;Cui Xiuming;Nian Hongjuan(Kunming Key Laboratory of Sustainable Development and Utilization of Famous-region Drug,Key Laboratory of Panax Notoginseng Resources Sustainable Development and Utilization of State Administration of Traditional Chinese Medicine,Kunming University of Science and Technology,Kunming,650500;Faculty of Agriculture and Food,University of Science and Technology,Kunming,650500)

机构地区：[1]昆明理工大学生命科学技术学院,云南省三七资源可持续发展利用重点实验室,国家中药管理局三七资源可持续发展利用研究室,昆明市道地药材可持续发展利用重点实验室,昆明650500 [2]昆明理工大学农业与食品学院,昆明650500

出　　处：《基因组学与应用生物学》2020年第3期1105-1112,共8页Genomics and Applied Biology

基　　金：国家自然科学基金(31560246,51779113)资助。

摘　　要：尖孢镰刀菌是导致三七根腐病的病原之一。本研究采用Illumina高通量测序技术对三七根腐病原菌(Fusarium oxysporum)的基因组扫描测序,并进行了基因组序列分析、基因功能注释和比较基因组学分析,从全基因组水平鉴定致病相关基因。扫描结果表明,基因组大小为48.12 Mb,G+C含量为51.5%,15 746个基因编码区(CDS),其中11 330个基因(71.95%)可以在COG数据库中得到其分类信息,编码有功能的蛋白6 022个。该序列已经提交GenBank数据库,登录号为SAMN09976373。进一步分析得到细胞壁降解相关基因45个,搜索数据库中F. oxysporum MAPK级联途径组分的基因序列,与酿酒酵母中MAPK级联途径进行比对并进行系统进化分析。研究结果对深入理解尖孢镰刀菌导致三七致病的分子机理,从而进一步有效控制病害的发生具有重要意义。Fusarium oxysporum is one of the pathogens causing Panax notoginseng root rot. In this study,high-throughput sequencing technology(Illumina) was used to sequence the genome of F. oxysporum. The genome sequence was analyzed, gene function was annotated and comparative genomics analysis was performed to identify pathogenic related genes from the whole genome level. The scan results showed that the genome size was 48.12 Mb, the G+C content was 51.5%, and the gene coding regions(CDS) were 15 746, of which 11 330 genes(71.95%) could obtain their classification information in the COG database and encode 6 022 functional proteins.This sequence has been submitted to the GenBank database under accession number SAMN09976373. Further analysis was conducted and 45 were predicted as cell wall degradation related genes. The gene sequence of the F. oxysporum MAPK cascade pathway was compared with the MAPK cascade pathway in Saccharomyces cerevisiae and phylogenetic analysis was performed. The results are of great significance for understanding the molecular mechanism of F. oxysporum causing P. notoginseng disease and further effectively controlling the occurrence of diseases.

关 键 词：三七 根腐病 尖孢镰刀菌 基因组 MAPK途径 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]