初花期和盛花期的桂花比较转录组分析  

Comparative Transcriptome Analysis of Osmanthus fragrans between Initial Flowering Stage and Full-blooming Stage

作　　者：鲜小林 潘远智[1] 陈睿[2] 宋海岩[2] Xian Xiaolin;Pan Yuanzhi;Chen Rui;Song Haiyan(College of Landscape Architecture,Sichuan Agricultural University,Chengdu,611130;Key Laboratory of Horticultural Crop Biology and Germplasm Creation in Southwestern China of theMinistry ofAgriculture and Rural Affairs,Sichuan Academy ofAgricultural Sciences,Chengdu,610066)

机构地区：[1]四川农业大学风景园林学院,成都611130 [2]四川省农业科学院园艺研究所,农业农村部西南地区园艺作物生物学与种质创制重点实验室,成都610066

出　　处：《基因组学与应用生物学》2020年第3期1208-1214,共7页Genomics and Applied Biology

基　　金：四川省财政专项资金项目(四川蔬菜花卉种质创新与产业提升关键技术研究专项(2016CYTS-010))资助。

摘　　要：桂花开放期间色泽和香味均发生明显变化,目前对于其内在的分子机制尚不清楚。本研究以四季桂"金玉台阁"为实验材料,对初花期和盛花期桂花花朵进行转录组测序。去除低质量数据后,初花期桂花文库得到6 342万条Clean reads,盛花期桂花文库得到4 941万条Clean reads,经de novo组装后共得到113 292条Unigenes,平均长度为775 bp。在设定阈值下(|log2 Ratio|≥1且FDR≤0.05),有1 381个Unigenes上调表达,464个Unigenes下调表达。Gene Ontology (GO)分析显示有47个GO类别被显著富集;KEGG分析显示有24代谢通路显著富集,涉及植物激素与信号传导、类胡萝卜素生物合成、萜类生物合成、苯丙氨酸代谢、淀粉与蔗糖代谢等代谢过程。此外,还在转录组数据中鉴定到9个类胡萝卜素合成基因和3个香味形成基因,大部分基因在盛花期的表达量要高于初花期。本研究有助于桂花花色和香味形成关键基因的挖掘,且对今后桂花遗传改良育种有一定的指导意义。While the big difference changes in colour and aroma among Osmanthus fragrans flowering, the underlying molecular mechanism is not yet clear. In this study, the transcriptome sequencing of initial flowering stage and full-blooming stage flower were performed, using Osmanthus fragrans cv ’Jin Yu Tai Ge’. After removing invalid data, 63.42 million and 49.41 million clean reads were generated in initial flowering stage library and full-blooming stage library, respectively. Finally a total of 113 292 unigenes were obtained by de novo assembly,with an average sequence length of 775 bp. Under the limited role(|log2 Ratio|≥1 and FDR≤0.05), 1 381 unigenes were up-regulated while 464 unigenes were down-regulated. Gene Ontology(GO) analysis showed 47 GO terms were significantly enriched, while Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis revealed24 biological pathways were notably enriched, including plant hormone signal transduction, carotenoid biosynthesis, terpenoid biosynthesis, phenylpropanoid metabolism, starch and sucrose metabolism, etc. Additionally,9 unigenes related to carotenoid biosynthesis and 3 unigenes related to aroma formation were identified in transcriptome data, and most of them expression level were higher in full-blooming stage than that in initial flowering stage. The research present here not only helps for the identification of colour and aroma related genes,but also provides guides for the molecular breeding in Osmanthus fragrans.

关 键 词：桂花 类胡萝卜素 香味 转录组 

分 类 号：S685.13[农业科学—观赏园艺]