Ca^2+介导肾小管ICAM-1高表达参与肾结石的形成  

Ca^2+ Mediates High Expression of ICAM-1 in Renal Tubules Involved in Renal Calculi Formation

作　　者：陈文炜 江涛[1] 何彦丰[1] 张华[1] 刘昌毅 高锐[1] 毛厚平[1] 陈沁[1] Chen Wenwei;Jiang Tao;He Yanfeng;Zhang Hua;Liu Changyi;Gao Rui;Mao Houping;Chen Qin(The Fisrt Affiliated Hospital of Fujian Medical University,Fuzhou,350005)

机构地区：[1]福建医科大学附属第一医院,福州350005

出　　处：《基因组学与应用生物学》2020年第3期1325-1330,共6页Genomics and Applied Biology

摘　　要：本研究旨在探讨细胞间黏附分子1 (intercellular cell adhesion molecule-1, ICAM-1)在高钙尿肾结石(genetic hypercalcium renal stones, GHS)大鼠中的表达以及Ca^2+对肾小管上皮细胞ICAM-1的影响。取GHS大鼠和SD大鼠,荧光定量PCR检测肾组织ICAM-1 mRNA表达水平,免疫组化检测ICAM-1蛋白表达。比色法检测大鼠肾组织SOD活力和MDA水平。通过ICAM-1 siRNA转染大鼠肾小管上皮细胞系NRK-52E构建ICAM-1低表达细胞模型,Ca^2+(5 mmol/L)处理NRK-52E细胞,检测细胞SOD活力和MDA水平,通过Western blotting检测细胞ICAM-1蛋白表达水平。荧光定量PCR结果显示,与SD对照组相比,GHS组大鼠肾组织ICAM-1 mRNA水平显著升高,差异具有统计学意义(p<0.01);免疫组化结果显示,ICAM-1蛋白在GHS大鼠肾组织中呈阳性表达;氧化应激检测结果显示,与SD对照组比较,GHS组大鼠肾组织SOD活性显著降低,MDA含量显著升高,差异具有统计学意义(p<0.01)。Western blotting结果显示,与对照组比较,Ca^2+组NRK-52E细胞ICAM-1表达蛋白显著升高,差异具有统计学意义(p<0.01);与Ca^2+处理NC-siRNA组比较,Ca^2+处理ICAM-1 siRNA组NRK-52E细胞ICAM-1表达蛋白显著降低;与ICAM-1 siRNA组NRK-52E细胞比较,Ca^2+处理ICAM-1 siRNA组NRK-52E细胞后ICAM-1表达蛋白水平无显著性变化(p>0.05)。细胞氧化应激检测结果显示,与对照组比较,Ca^2+组NRK-52E细胞SOD活性显著降低,MDA含量显著升高,差异具有统计学意义(p<0.01);与Ca^2+处理NC-siRNA组比较,Ca^2+处理ICAM-1 siRNA组SOD活性显著升高,MDA含量显著降低,差异均具有统计学意义(p<0.01);与ICAM-1 siRNA组NRK-52E细胞比较,Ca^2+处理ICAM-1 siRNA组NRK-52E细胞SOD活力和MDA含量无显著性变化(p>0.05)。ICAM-1在GHS肾小管上皮细胞中高表达,Ca^2+诱导肾小管上皮细胞ICAM-1高表达,促进细胞氧化应激水平。The aim of this study was to investigate the expression of intercellular cell adhesion molecule-1(ICAM-1) in high-calcium renal stones(GHS) rats and the effect of Ca^2+ on ICAM-1 in renal tubular epithelial cells.The expression of ICAM-1 m RNA and protein in renal tissues of GHS rats and SD rats was detected by real-time PCR and immunohisto-chemistry. The SOD activity and MDA level of renal tissue in GHS rats and SD rats were detected. The ICAM-1 low expression cell model was constructed by transfecting rat renal tubular epithelial cell line NRK-52 E with ICAM-1 si RNA. The NRK-52 E cells were treated with Ca^2+(5 mmol/L), and the SOD activity and MDA level were detected, and the expression level of ICAM-1 protein was detected by Western blotting. The results of real-time PCR showed that the level of ICAM-1 m RNA in the renal tissue of the GHS group was significantly higher than that of the SD control group, and the difference was statistically significant(p<0.01). Immunohistochemistry showed that ICAM-1 protein was highly expressed in renal tissues of GHS rats. Compared with the SD control group, the SOD activity of the renal tissue of the GHS group was significantly decreased, and the MDA content was significantly increased, the difference was statistically significant(p<0.01). Western blotting results showed that compared with the control group, the expression of ICAM-1 in NRK-52E cells was significantly increased in the Ca^2+group, and the difference was statistically significant(p<0.01). Compared with Ca^2+-treated NC-siRNA group, the expression of ICAM-1 in NRK-52 E cells was significantly decreased in Ca^2+-treated ICAM-1 siRNA group(p<0.01). Compared with the ICAM-1 siRNA group, there was no significant change in the expression of ICAM-1 protein in NRK-52E cells in the Ca^2+-treated ICAM-1 siRNA group(p>0.05). The results of oxidative stress showed that compared with the control group, the SOD activity of NRK-52E cells in Ca^2+group was significantly decreased, and the MDA content was significantly increa

关 键 词：肾结石 ICAM-1 钙离子 氧化应激 

分 类 号：R692.4[医药卫生—泌尿科学]