溪黄草中迷迭香酸的含量测定研究  

Content determination of rosmarinic acid in Isodon striatus

作　　者：王清[1] 曹雨诞[2] 陈佩东[2] WANG Qing;CAO Yudan;CHEN Peidong(Department of Pharmacy,Jiangsu Province Hospital of Chinese Medicine,Nanjing,Jiangsu 210029,China;College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing,Jiangsu 210046,China)

机构地区：[1]江苏省中医院药剂科,江苏南京210029 [2]南京中医药大学药学院,江苏南京210046

出　　处：《安徽医药》2020年第6期1098-1101,共4页Anhui Medical and Pharmaceutical Journal

基　　金：江苏省药学会课题(苏食药监法科[2014]337号)。

摘　　要：目的测定溪黄草中迷迭香酸的含量。方法采用高效液相色谱-质谱法(HPLC-MS)分析,并应用高效液相色谱(HPLC)法测定溪黄草饮片中迷迭香酸含量,应用Kromasil C1(8250 mm×4.6 mm,5μm)色谱柱,以乙腈、0.1%磷酸(25∶75)洗脱并进行方法学考察。结果迷迭香酸在0.063~1.012μg/mL,r=0.9997范围内呈良好的线性关系,迷迭香酸的平均回收率为96.40%,相对标准偏差(RSD)为2.46%。结论该研究所建立的测定方法简便、重现性好,可用于溪黄草的质量控制。Objective To determine the content of rosmarinic acid in Isodon striatus.Methods The content of rosmarinic acid was analyzed by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS)and measured by high performance liquid chromatography(HPLC).A Kromasil C18(250 mm×4.6 mm,5μm)column was adopted.Acetonitrile and 0.1% phosphoric acid(25∶75)was used for elution and a methodological investigation was conducted.Results Rosmarinic acid showed good linearity within the range of 0.063-1.012μg/mL,r=0.9997.The average recovery rate was 96.40% and relative standard deviation(RSD)was 2.46%.Conclusion The method is convenient and repeatable,which is suitable for the quality control of Isodon striatus.

关 键 词：溪黄草/分析 回收率 色谱法 高压液相 含量测定 迷迭香酸 

分 类 号：R284.1[医药卫生—中药学]