HPLC波长切换法同时测定沉香舒气丸中9种成分的含量  

Simultaneous Determination of Nine Compositions in Chenxiang Shuqi Wan by HPLC Wavelength Switching Method

作　　者：林源 张成中 LIN Yuan;ZHANG Chengzhong(Wenling Hospital of Traditional Chinese Medicine,Wenling 317500,China;Fuyang Institute for Food and Drug Control,Fuyang 236015,China)

机构地区：[1]温岭市中医院,浙江温岭317500 [2]阜阳市食品药品检验检测中心,安徽阜阳236015

出　　处：《中国现代应用药学》2020年第5期582-586,共5页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立并验证同时测定沉香舒气丸中α-香附酮、木香烃内酯、去氢木香内酯、原阿片碱、延胡索乙素、紫堇碱、四氢小檗碱、和厚朴酚、厚朴酚含量的HPLC波长切换方法。方法采用Kromasil Eternity C18色谱柱(250 mm×4.6 mm,5μm);流动相A为甲醇-乙腈(1∶1),流动相B为0.1%磷酸溶液,梯度洗脱;检测波长分别为250,225,280,294 nm;体积流量0.9 mL·min-1;柱温30℃。结果 9种成分α-香附酮、木香烃内酯、去氢木香内酯、原阿片碱、延胡索乙素、紫堇碱、四氢小檗碱、和厚朴酚、厚朴酚分别在5.79~115.80,7.86~157.20,9.68~193.60,1.07~21.40,3.09~61.80,3.77~75.40,2.09~41.80,15.77~315.40,19.86~397.20μg·mL-1内线性关系良好;平均加样回收率分别为97.95%,98.97%,99.46%,97.37%,98.48%,98.10%,96.90%,99.35%和100.10%,RSD分别为1.35%,1.17%,0.89%,1.57%,1.27%,0.96%,1.09%,0.74%和0.90%。结论所建立的测定方法操作便捷、重复性好,可用于沉香舒气丸的质量控制。OBJECTIVE To develop and validate an HPLC wavelength switching method for detemining contents of α-cyperone, costunolide, dehydrocostuslactone, protopine, tetrahydropalmatine, corydaline, tetrahydroberberine, honokiol and magnolol in Chenxiang Shuqi Wan. METHODS HPLC method was employed on a Kromasil Eternity C18 chromatographic column(250 mm×4.6 mm, 5 μm). The mobile phase was methanol-acetonitrile(1∶1)(A)-0.1% phosphoric acid solution(B) with gradient elution. The detection wavelengths were 250, 225, 280, 294 nm. The flow rate was 0.9 mL·min-1, and the column temperature was 30 ℃. RESULTS The liner ranges of α-cyperone, costunolide, dehydrocostuslactone, protopine, tetrahydropalmatine, corydaline, tetrahydroberberine, honokiol and magnolol were within 5.79-115.80, 7.86-157.20, 9.68-193.60, 1.07-21.40, 3.09-61.80, 3.77-75.40, 2.09-41.80, 15.77-315.40, 19.86-397.20 μg·mL-1. The average recovery rates were 97.95%, 98.97%, 99.46%, 97.37%, 98.48%, 98.10%, 96.90%, 99.35% and 100.10%, with RSD of 1.35%, 1.17%, 0.89%, 1.57%, 1.27%, 0.96%, 1.09%, 0.74% and 0.90%. CONCLUSION The method was easy to operate and repeatable, can be used for quality control of Chenxiang Shuqi Wan.

关 键 词：沉香舒气丸 波长切换法 高效液相色谱法 化学成分 

分 类 号：R284.1[医药卫生—中药学]