miRNA-499多态性对桥本甲状腺炎易感性的影响  

Effect of polymorphism in miRNA-499 on susceptibility of Hashimoto′s thyroiditis

作　　者：吴平平 章燕[2] 黄海华[2] 甘华侠[2] WU Pingping;ZHANG Yan;HUANG Haihua;GAN Huaxia(Department of Dermatology,the Affiliated Hospital of Jiujiang University,Jiujiang,Jiangxi 332000,China;Department of Endocrinology,the First Affiliated Hospital of Nanchang University,Nanchang,Jiangxi 330006,China)

机构地区：[1]九江学院附属医院皮肤科,江西九江332000 [2]南昌大学第一附属医院内分泌科,南昌330006

出　　处：《重庆医学》2020年第10期1589-1592,共4页Chongqing medicine

基　　金：江西省重点研发计划项目(20161BBG70179)。

摘　　要：目的探讨miRNA-499(rs3746444)单核苷酸多态性(SNPs)与中国江西地区汉族人群桥本甲状腺炎(HT)发病的相关性。方法选取江西本地汉族HT患者(观察组,n=96)及同期健康者(对照组,n=80)为研究对象,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测miRNA-499基因多态性,分析比较不同基因型的分布频率及对疾病严重程度的影响。结果观察组及对照组的miRNA-499(rs3746444)均存在T/T、T/C、C/C 3种基因型,基因型分布频率差异无统计学意义(P>0.05);T/C vs.T/T基因型分布频率差异有统计学意义(P<0.05);T、C等位基因分布频率在两组间的差异无统计学意义(P>0.05);TPOAb≥600U/mL及TPOAb<600U/mL两亚组间基因型分布频率及等位基因分布频率比较,差异无统计学意义(P>0.05)。结论miRNA-499T/C基因型可能是HT发病的风险因素,miRNA-499多态性与疾病严重程度无关。Objective To investigate the association of single nucleotide polymorphisms(SNPs)in miRNA-499(rs3746444)with Hashimoto′s thyroid disease(HT)susceptibility among Chinese Han population in Jiangxi.Methods HT patients(the experiment group,n=96)and healthy people(the control group,n=80)in Jiangxi Han population were selected as subjects.The single nucleotide polymorphisms(SNPs)in miRNA-499(rs3746444)was genotyped by polymerase chain reaction-restriction fragment length polymorphism(PCRRFLP).The distribution frequency of genetic polymorphisms in miRNA-499 and the effects on disease severity were analyzed and compared.Results There were three genotypes of miRNA-499(rs3746444)polymorphism T/T,T/C and C/C in both the experiment group and the control group,there was no significant difference in distribution frequency between different genotypes(P>0.05).The distribution frequency between the genotypes T/C and T/T had statistically significant differences(P<0.05),but there was no statistically significance between the frequency of allele C and T(P>0.05).The genotype distribution frequency and allele distribution frequency between the TPOAb≥600 U/mL group and the TPOAb<600 U/mL group had no statistically significance(P>0.05).Conclusion The genotype T/C of miRNA-499(rs3746444)may be a risk factor for HT.miRNA-499 polymorphism was not associated with the disease severity.

关 键 词：微RNAS 桥本病 单核苷酸多态性 

分 类 号：R581.4[医药卫生—内分泌]