UCP2基因多态性与噪声性听力损失易感性的关系  

Relationship between UCP2 gene polymorphism and susceptibility of noise-induced hearing loss

作　　者：王科 朱云[2] 王应强 马世丽[3] 叶静 WANG Ke;ZHU Yun;WANG Yingqiang;MA Shili;YE Jing(Department of ORL-HNS,363 Hospital,Chengdu,Sichuan 610000,China;the First Affiliated Hospital of Medical College of Xi'an Jiaotong University,Xi'an,Shaanxi 710061,China;Department of Otolaryngology,Chengdu First people's Hospital,Chengdu,Sichuan 610041,China)

机构地区：[1]三六三医院耳鼻喉头颈外科,成都610000 [2]西安交通大学医学院第一附属医院,西安710061 [3]成都市第一人民医院耳鼻喉科,610041

出　　处：《重庆医学》2020年第10期1643-1647,1652,共6页Chongqing medicine

摘　　要：目的探究解偶联蛋白2(UCP2)基因多态性与噪声性听力损失(NIHL)易感性的关系。方法选择三六三医院耳鼻喉头颈外科体检的某工厂319例噪声作业工人作为研究对象,根据听力检测结果分为NIHL组(163例)与噪声性听力未损伤组(对照组,156例),所有受试者均进行问卷调查及纯音听力检测,采集静脉血,采用聚合酶链式反应-限制性片段多态性(PCR-RFLP)法检测血液中UCP2基因A55V位点、886G/A位点基因分型,Logistic多因素分析UCP2基因分型与噪声性听力损失发生风险的关系;分析各基因型与临床参数的关系。结果NIHL组高频听阈值高于对照组,差异有统计学意义(P<0.05)。两组UCP2基因A55V位点、886G/A位点基因频率实际值、理论值对比,差异无统计学意义(P>0.05),两组符合Hardy-Weinberg遗传平衡定律。两组A55V位点基因CC、TC、TT型基因频率比较差异有统计学意义(P<0.05),NIHL组T等位基因频率高于对照组,差异有统计学意义(P<0.05)。UCP2基因A55V基因分型与高频听阈相关(P<0.05)。Logistic多因素回归分析显示UCP2基因A55V位点T等位基因携带者发生声性听力损失的危险度为C等位基因的1.963倍(P<0.05)。结论UCP2基因A55V位点多态性为NIHL易感性的危险因素,A55V位点C→T突变可能会增加NIHL易感性。Objective To explore the relationship between uncoupling protein 2(UCP2)gene polymorphism and the susceptibility of noise-induced hearing loss(NIHL).Methods A total of 319 workers exposed to noise in a factory were selected as the research objects,according to the results of hearing test,they were divided into the NIHL group(163 cases)and the noise-induced hearing impairment group(the control group,156 cases).Questionnaires and pure tone audiometry were conducted in all subjects,and venous blood was collected,polymerase chain reaction-restriction fragment polymorphism(PCR-RFLP)was used to detect the genotyping of A55 Vand 886 G/A locus of UCP2 gene in blood.Logistic multivariate analysis was used to analyze the relationship between UCP2 genotyping and the risk of noise-induced hearing loss,and the relationship between genotypes and clinical parameters was analyzed.Results The threshold of high frequency hearing in the NIHL group was higher than that in the control group(P<0.05).There was no significant difference in the actual and theoretical values of gene frequency A55 Vand 886 G/A locus of UCP2 between the two groups(P>0.05).The two groups accorded with Hardy-Weinberg′s law of genetic balance.The frequencies of CC,TC and TT genotypes at A55 Vlocus were significantly different between the two groups,and the frequencies of T alleles in the NIHL group were significantly higher than those in control group(P<0.05).The A55 V genotype of UCP2 gene was correlated with high frequency hearing threshold(P<0.05).Logistic multivariate regression analysis showed that the risk of acoustic hearing loss in carriers of T allele at A55 Vlocus of UCP2 gene was 1.963 times higher than that of C allele(P<0.05).Conclusion The A55 Vpolymorphism of UCP2 gene is a risk factor for the susceptibility of noise-induced hearing loss,and the C→T mutation of A55 V locus may increase the susceptibility of noise-induced hearing loss.

关 键 词：解偶联蛋白2 噪声性听力损失 基因多态性 风险分析 

分 类 号：R765.4[医药卫生—耳鼻咽喉科]