急性肺血栓栓塞症的非靶向代谢组学研究  

Plasma biomarkers research on patients with acute pulmonary thromboembolism based on lc-ms analysis

作　　者：王静[1,2] 尤宏钊[2] 杨新颖 郭伟 WANG Jing;YU Hongzhao;YANG Xinying;GUO Wei(Hospital,Capital Medical University,Beijing 100070,China)

机构地区：[1]首都医科大学附属北京天坛医院急诊科,100071 [2]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所心血管生物研究室

出　　处：《心肺血管病杂志》2020年第5期570-576,共7页Journal of Cardiovascular and Pulmonary Diseases

摘　　要：目的:本研究基于液相色谱质谱(LC-MS)生物学技术对急性肺血栓栓塞症(PTE)患者血清样本进行非靶向代谢组学研究,筛选出一批与PTE诊断密切相关的潜在生物标志物,作为PTE早期诊断的潜在生物标志物的识别。方法:纳入2016年1月至12月,于北京安贞医院诊治的急性PTE患者28例,同时期健康对照组28例。所有患者均记录基本临床资料、生命体征、血常规、血生化指标,同时收集静脉血浆,采用LC-MS和Multiquenti分析软件分析代谢产物的变化。结果:与对照组相比较,PTE患者瓜氨酸、缬氨酸、乙酰乙酸、天冬氨酸、维生素B12、甘油磷脂、天冬氨酸等代谢产物改变明显,差异有统计学意义(P<0.05)。与对照组相比较,PTE组患者在糖代谢、三羧酸循环代谢、甘油磷脂代谢等代谢途径,差异有统计学意义(P<0.05)。结论:急性PTE患者机体瓜氨酸等代谢产物发生改变,相应的代谢通路明显变化,为急诊快速识别PTE提供诊断思路。Objective:To analysis the changes of metabolites in patients of acute pulmonary thrombo-embolism(PTE).Methods:PTE patients(n=28)and healthy control peop1e(n=28)were chosen from Beijing Anzhen Hospital from Jan.2016 to Nov.2016.A11 patients5 blood samples were collected.The changes of metabolites were analyzed by LC-MS.Principal Component analysis and Partial least square-discriminant analysis were used to screen the plasma differential metabolites of PTE patients and control group,using the Meta Analyst platform analyze the possible metabolic pathways.Results:Clear distinction between the groups,different metabolites and metabolic pathways can be identified.The changes of Citrulline,valine,acetoacetate,Aspartic acid,vitamin B12,glycerophospholipid were more significant in PTE patients compared with control(P<0.05).The changes of Glycometabolism,tricarboxylic acid cycle metabolism,glycerol phospholipid metabolism pathway were significant in PTE patients compared with control(P<0.05).Conclusions:The body metabolism,metabolites and relevant metabolic pathways will change in PTE patients.

关 键 词：急性肺血栓栓塞症 代谢组学 液相色谱质谱 代谢产物 代谢通路 

分 类 号：R54[医药卫生—心血管疾病]