LNK在高血压血管重构中的作用研究  

The role of LNK in hypertension vascular remodeling

作　　者：邵明明 阿燕·西合斯 王凌鹏[2] 罗健[3] SHAO Ming-ming;AYAN-Xihesi;WANG Ling-peng;LUO Jian(The First Clinical Medical College of Xinjiang Medical University,Urumqi 830054,China;Department of Heart Failure,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China;Department of General Internal Medicine,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一临床医学院,新疆维吾尔自治区乌鲁木齐市830054 [2]新疆医科大学第一附属医院心力衰竭科,新疆维吾尔自治区乌鲁木齐市830054 [3]新疆医科大学第一附属医院综合内医科,新疆维吾尔自治区乌鲁木齐市830054

出　　处：《中国心血管病研究》2020年第5期441-445,共5页Chinese Journal of Cardiovascular Research

基　　金：新疆维吾尔自治区科技支疆项目(2017E0268)。

摘　　要：目的探讨LNK在高血压血管重构中的作用.方法选取大鼠胸主动脉平滑肌细胞,在一定条件下构建高血压细胞模型.根据不同处理方法分为空白对照组,AngⅡ+空白对照组,AngⅡ+空载组和AngⅡ+SH2B3重组载体组.流式细胞仪检测VSMC周期、凋亡及增殖的水平;ELISA检测IL-6、TNF-α、IFN-γ的表达水平.结果①各组大鼠血管平滑肌细胞周期、凋亡及增殖的水平:与空白对照组比较,AngⅡ+空白对照组和AngⅡ+空载组VSMC处于G0/G1期减少[(71.357±0.312)%比(67.480±0.613)%、(67.053±0.321)%],S期增加[(21.517±0.254)%比(24.003±0.748)%、(22.927±0.178)%],G2/M期增加[(7.137±0.159)%比(8.483±0.618)%、(10.020±0.364)%],差异有统计学意义(P<0.05).与AngⅡ+空载组比较,AngⅡ+SH2B3重组载体组VSMC处于G0/G1期增加[(67.053±0.321)%比(70.810±1.470)%],S期减少[(22.927±0.178)%比(21.867±0.676)%],G2/M期减少[(10.020±0.364)%比(7.320±1.002)%],差异有统计学意义(P<0.05).AngⅡ+SH2B3重组载体组VSMC细胞凋亡率高于AngⅡ+空白对照组和AngⅡ+空载组[(5.210±0.053)%比(4.503±0.223)%、(3.837±0.546)%](P<0.05).AngⅡ+SH2B3重组载体组VSMC细胞增殖水平低于AngⅡ+空白对照组和AngⅡ+空载组[(1.263±0.053)比(1.482±0.104)、(1.480±0.092)](P<0.05).②各组大鼠血管平滑肌细胞上炎性细胞因子水平:与空白对照组相比,AngⅡ+空白对照组和AngⅡ+空载组IL-6[(176.023±16.776)pg/ml比(264.560±12.368)pg/ml、(486.147±41.893)pg/ml]、TNF-α[(8.563±0.667)pg/ml比(15.738±0.995)pg/ml、(20.389±1.274)pg/ml]和IFN-γ[(631.117±26.551)pg/ml比(1097.271±32.303)pg/ml、(1207.185±38.949)pg/ml]水平均升高,AngⅡ+SH2B3重组载体组IL-6[(157.410±12.456)pg/ml]和IFN-γ[(723.270±15.317)pg/ml]表达降低,差异具有统计学意义(P<0.05).与AngⅡ+空白对照组和AngⅡ+空载组相比,AngⅡ+SH2B3重组载体组的IL-6[(264.560±12.368)pg/ml、(486.147±41.893)pg/ml比(157.410±12.456)pg/ml]、TNF-α[(15.738±0.995)pg/ml、(20.389±1.274)pg/mObjective To investigate the role of LNK in vascular remodeling in hypertension.Methods Rat thoracic aorta smooth muscle cells were selected to construct the hypertensive cell model under certain conditions.According to the different processing methods were divided into blank control group,Aug H+blank group,AngⅡ+light group and AngⅡ+LNK recombinant plasmid group.The cycle,apoptosis and proliferation of VSMC were measured by flow cytometry.The expression levels of IL-6,TNF-αand IFN-γwere detected by ELISA.Results(1)Each group's rat vascular smooth muscle cell cycle,apoptosis and proliferation of level:Compared with blank control group,VSMC of Ang H+blank group and AngⅡ4-light group were in G0/G1 phase decreased[(71.357±0.312)%vs.(67.480±0.613)%and(67.053±0.321)%],S phase increased[(21.517土0.254)%vs.(24.003±0.748)%and(22.927±0.178)%]and G2/Mphase increased[(7.137±0.159)%vs.(8.483土0.618)%and(10.020±0.364)%],statistically significant difference(P<0.05).Compared with Ang U 4-light group,VSMC of AngⅡ+LNK recombinant plasmid group in G0/G1 phase increased[(67.053±0.321)%vs.(70.810±1.470)%],S phase decreases[(22.927±0.178)%vs.(21.867±0.676)%],G2/M phase decreases[(10.020±0.364)%vs.(7.320±1.002)%],statistically significant difference(P<0.05);VSMC apoptosis of Ang Ⅱ+LNK recombinant plasmid group rate was higher than AngⅡ+blank group and Ang H+light group[(5.210±0.053)%vs.(4.503±0.223)%and(3.837±0.546)%](P<0.05).AngⅡ+LNK recombinant plasmid group of VSMC cell proliferation levels below AngⅡ+blank group and AngⅡ+light group[(1.263±0.053)vs.(1.482土0.104)and(1.480±0.092)],statistically significant difference(P<0.05).⑵Each rat vascular smooth muscle cells on level of inflammatory cytokines:Compared with the blank control group,Ang Ⅱ+blank group and Ang H+light group IL-6[(176.023±16.776)pg/ml vs.(264.560±12.368)pg/ml and(486.147±41.893)pg/ml],TNF-oc[(&563±0.667)pg/ml vs.(15.738±0.995)pg/ml and(20.389±1.274)pg/ml],IFN-γ[(631.117±26.551)pg/ml vs.(1097.271±32.303)pg/ml and(1

关 键 词：高血压 血管重构 血管平滑肌细胞 LNK 

分 类 号：Q95-33[生物学—动物学] R544.1[医药卫生—心血管疾病]