FGM发酵黄芪液安全性初步评价  

Preliminary Safety Evaluation of the Fermented Astragalus Liquid by FGM Strain

作　　者：王恒 张宏 张凯[3] 王磊[3] 张康[3] 马玉俊 李建喜[3] 杨孝朴[1] 张景艳[1,3] WANG Heng;ZHANG Hong;ZHANG Kai;WANG Lei;ZHANG Kang;MA Yu-jun;LI Jian-xi;YANG Xiao-pu;ZHANG Jing-yan(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;The monitoring institute of Veterinary drug and feed of Gansu province,Lanzhou 730000,China;Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS,Lanzhou 730050,China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]甘肃省兽药饲料监察所,兰州730000 [3]中国农业科学院兰州畜牧与兽药研究所,兰州730050

出　　处：《中国兽药杂志》2020年第5期49-56,共8页Chinese Journal of Veterinary Drug

基　　金：国家自然科学基金项目(31602101);中国农业科学院基本科研业务费专项所级统筹项目(1610322020013);中国农业科学兰州畜牧与兽药研究所科技创新工程所级重点任务(CAAS-LMY-02);甘肃省农业生物技术研究与应用开发项目(GNSW-2016-12)。

摘　　要：为了评价FGM发酵黄芪液的安全性,本实验用FGM菌发酵黄芪液及其发酵菌株FGM菌悬液经口灌胃进行小鼠急性毒性试验,试验分为FGM活菌组(FGM)、FGM发酵液组(FAL)、阴性对照组(NG),分别连续灌胃3 d,每天2次,7 d后处死小鼠,解剖观察。结果显示,试验各组小鼠无中毒反应和死亡。依据小鼠一般体征评价,各组试验小鼠精神状态良好,食欲正常,可判定为Ⅰ级。解剖小鼠后,脏器未发现病变;FGM组、FAL组与阴性对照组相比,小鼠体重,脏器指数、血液指标无显著性差异(P>0.05);对主要脏器病理组织切片显微观察,各组小鼠无明显的病理变化。研究表明,FGM菌株及其发酵黄芪液饲喂小鼠,无毒副作用和不良反应。In order to evaluate the safety of the fermented Astragalus liquid by FGM strain,The mice were divided into 3 groups which include FGM group(FC),the fermented Astragalus liquid group(FAL)and negative control group(NC),were administrated orally with Astragalus fermentation,FGM stain and normal saline.The mice were sacrificed after 7 days of continuous gavages for anatomical observation.The results showed the mice in all groups didn’t have any toxic reaction and death.According to the standard of general health appearance score of mice,the mental state and appetite of tested mice was normal,and the health level of tested mice was judged asⅠlevel.There were no obvious pathological changes for anatomic observation on mice,and no significant difference in body weight,viscera index and blood index between FAL,FC and NC(P>0.05).The histological sections of the main organs were observed microscopically without obvious pathological changes.The test showed that the fermented Astragalus liquid and its fermentation strains don’t have no side effects and adverse reactions on mice as feed additive.

关 键 词：发酵黄芪液 FGM菌株 安全性 

分 类 号：S853.7[农业科学—临床兽医学]