基于UPLC特征图谱及萜类成分含量测定的泽泻产地差异研究  

Regional differences analysis of Alismatis Rhizoma based on UPLC characteristic chromatogram and determination of eight terpenoids

作　　者：田双双 赵晓梅[1] 刘珊珊 冯伟红[1] 陈两绵[1] 闫利华[1] 王智民[1] TIAN Shuang-shuang;ZHAO Xiao-mei;LIU Shan-shan;FENG Wei-hong;CHEN Liang-mian;YAN Li-hua;WANG Zhi-min(National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Henan University of Chinese Medicine,Zhengzhou 450000,China)

机构地区：[1]中国中医科学院中药研究所中药质量控制技术国家工程实验室,北京100700 [2]河南中医药大学,河南郑州450000

出　　处：《中国中药杂志》2020年第7期1545-1557,共13页China Journal of Chinese Materia Medica

基　　金：国家中药标准化项目(ZYBZH-C-JL-24);国家药典委员会标准制修订研究课题(2018Z079)。

摘　　要：为了比较不同产地泽泻的质量差异,采用超高效液相色谱法,对37批泽泻药材与30批泽泻饮片进行测定,分别建立其特征图谱,并测定其中8个萜类成分的质量分数。采用相似度评价、主成分分析(PCA)和偏最小二乘法判别分析(PLS-DA)对样品数据进行分析。泽泻特征图谱中呈现3个主要特征峰,以23-乙酰泽泻醇B(S)为参照物峰,泽泻烯醇和泽泻醇B的相对保留时间分别为0.55,0.77。利用泽泻醇B与23-乙酰泽泻醇B的峰面积及其比值可以区分泽泻的产地。含量测定及PCA和PLS-DA结果表明,泽泻样品按产地可明显分成"建泽泻"(福建泽泻、江西泽泻)、"川泽泻"(四川泽泻、湖北泽泻)和"广泽泻"(广西泽泻)3类。不同产地泽泻中各成分含量差异具有统计学意义,泽泻醇A和24-乙酰泽泻醇A在"广泽泻"中的含量显著高于"建泽泻"和"川泽泻"(P<0.000 1);"川泽泻"中泽泻醇B、泽泻醇C等成分的含量显著高于"建泽泻"(P<0.000 1)。该研究建立的特征图谱结合萜类成分含量测定结果显示,不同产地泽泻化学成分的含量及比例关系存在显著差异,但成分的数目和种类基本一致。研究结果阐明了泽泻的产地差异及其成分特征,可为泽泻药材、饮片的鉴别及其质量控制提供参考依据。An ultra-performance liquid chromatography(UPLC) method integrating characteristic chromatogram and eight terpenoids determination has been established for comparing the differences of Alismatis Rhizoma(Zexie) from different product areas. Thirty-seven batches of crude drugs and thirty batches of prepared slices of Alismatis Rhizoma were analyzed. The obtained data were analyzed by similarity evaluation, principal components analysis(PCA) and partial least squares discriminant analysis(PLS-DA). There were three main characteristic peaks in the characteristic chromatograms, and alisol B 23-acetate(S) was selected as the reference. Compared with the S peak, the relative retention times of the other two characteristic peaks were 0.55(alisol) and 0.77(alisol B), respectively. Peak areas and the ratio of alisol B to alisol B 23-acetate could be used to distinguish Alismatis Rhizoma from different geographical origins. The samples were divided into three groups by PCA and PLS-DA based on the content determination results, and they were "Jian Zexie"(Fujian and Jiangxi provinces), "Chuan Zexie"(Sichuan and Hubei provinces), and "Guang Zexie"(Guangxi province). The contents of chemical components in samples from different producing areas were notably different. For example, the contents of alisol A and alisol A 24-acetate were significantly higher in "Guang Zexie" compared with "Jian Zexie" and "Chuan Zexie"(P<0.000 1). The contents of alisol B and alisol C were significantly higher in "Chuan Zexie" compared with "Jian Zexie"(P<0.000 1). Combining the characteristic chromatograms and quantitative analysis of eight terpenoids, this study showed that the relative contents of components and their ratios were notable different in samples from different regions, but types and numbers of chemical compositions were basically similar. The results of this study illustrated the regional differences of Alismatis Rhizoma and their components characteristics, and provided references for authentication and quality control of Alismatis

关 键 词：泽泻 三萜 倍半萜 超高效液相色谱 特征图谱 含量测定 

分 类 号：O657.72[理学—分析化学] R284.1[理学—化学]