MicroRNA-146参与碘诱导NOD.H-2h4小鼠自身免疫性甲状腺炎  

MicroRNA-146 contributes to iodine-induced autoimmune thyroiditis of NOD.H-2h4 mice

作　　者：赵娜[1] 赵姝洁[1] 李玉姝[1] ZHAO Na;ZHAO Shu-Jie;LI Yu-Shu(Department of Endocrinology and Metabolism,Institute of Endocrinology,Liaoning Provincial Key Laboratory of Endocrine Diseases,the First Affiliated Hospital of China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院内分泌科,内分泌研究所,辽宁省内分泌疾病重点实验室,沈阳110001

出　　处：《中国免疫学杂志》2020年第10期1159-1162,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金面上项目(No.81471003、No.81770784)。

摘　　要：目的:观察自身免疫性甲状腺炎(AIT)NOD.H-2h4小鼠microRNA-146(miR-146)的表达水平。方法:选取4周龄NOD.H-2h4雌性小鼠随机分为正常饮水(无菌双蒸水)组和高碘饮水(0.05%碘化钠水)组。甲状腺HE染色观察淋巴细胞浸润情况;获取CD4^+ T细胞及甲状腺组织small RNAs,RT-PCR检测miR-146表达水平;检测心、肝和肾中miR-146的表达水平。结果:高碘饮水组NOD.H-2h4小鼠甲状腺组织有不同程度的淋巴细胞浸润,甲状腺炎的发生率均高于对照组。与对照组相比,高碘饮水组小鼠脾脏CD4^+ T细胞和甲状腺中miR-146水平显著升高。高碘饮水组小鼠与对照组相比,心、肝和肾中miR-146的水平没有明显差别。结论:在NOD.H-2h4小鼠AIT模型中,甲状腺和脾脏CD4^+ T miR-146水平升高,可能参与自身免疫性甲状腺炎的发生和发展。Objective:To observe levels of miR-146 in NOD.H-2 h4 mice with iodine-induced autoimmune thyroiditis.Methods:Female NOD.H-2 h4 mice at 4 weeks were randomly divided into high-iodine group(0.05% NaI) and control group(sterile double distilled water).Thyroid sections were stained with HE assay.CD4^+T cells were collected by MACS.Small RNAs of CD4^+T cells and thyroid tissues were prepared for evaluating the levels of miR-146 by RT-PCR.The levels of miR-146 in heart,liver and kidney were also analyzed.Results:The present study found that the levels of miRNA-146 in CD4^+T cells and thyroid tissues of high-iodine group increased significantly than control group,while no difference of the levels of miRNA-146 in heart,liver and kidney was detected.Conclusion:MiR-146 may involve in the development of autoimmune thyroiditis in NOD.H-2 h4 mice.

关 键 词：自身免疫性甲状腺炎 miR-146 CD4^+T细胞 NOD.H-2h4小鼠 

分 类 号：R581.4[医药卫生—内分泌]