鱼腥草素钠对哮喘小鼠血清TNF-α和IL-4水平的影响研究  

Effect of Houttuynia Sodium on Serum TNF-αand IL-4 Levels in Asthmatic Mice

作　　者：黄丽娟 陈叶 胡智立 HUANG Li-juan;CHEN Ye;HU Zhi-li(Union Hospital Shenzhen Hospital,Huazhong University of Science and Technology,Shenzhen,Guangdong Province,518000 China)

机构地区：[1]华中科技大学协和深圳医院,广东深圳518000

出　　处：《系统医学》2020年第6期23-25,共3页Systems Medicine

基　　金：深圳市南山区科技创新资金项目(南科研卫2017016)。

摘　　要：目的观察鱼腥草素钠对哮喘小鼠血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-4(IL-4)含量的影响,探讨鱼腥草素钠抑制哮喘气道重塑的作用机制。方法将30只BALA/c小鼠随机分为正常对照组(8只)、模型组(11只)、鱼腥草素钠组(11只),灌胃(ig)给药2周,对照组和模型组给予等体积生理盐水。采用小鼠肺功能仪器检测小鼠气道高反应性。用酶联免疫吸附试验(ELISA)法检测血清中肿瘤坏死因子TNF-α、白细胞介素-4(IL-4)的含量。结果模型组、鱼腥草素钠组小鼠血清中TNF-α、IL-4与正常组比较含量升高,差异有统计学意义(P<0.05);鱼腥草素钠组血清中TNF-α、IL-4与模型组比较含量降低[(1198.7±261.6)ng/L,(36.2±2.34)pg/mL vs(1310.1±271.1)ng/L,(53.5±2.58)pg/mL],差异有统计学意义(P<0.05)。结论鱼腥草素钠可有效降低哮喘小鼠血清中TNF-α、IL-4的含量,其机制可能是鱼腥草素钠通过降低小鼠体内TNF-α、IL-4的含量抑制NF-κB信号通路的活性从而发挥哮喘的治疗作用。Objective To observe the effect of sodium houttuyfonate on the levels of TNF-αand IL-4 in serum of asthma mice and to explore the mechanism of sodium houttuyfonate in inhibiting airway remodeling in asthma.Methods Thirty BALB/c mice randomly divided into normal control group(n=8),model group(n=11),sodium houttuyfonate group(n=11).After 2 weeks of irrigation(ig)administration,the control group and model group were given normal saline of equal volume.The airway hypersponsiveness of mice was measured by pulmonary function instrument,the tumor necrosis factor-alpha(TNF-α)and interleukin-4(IL-4)levels in serum were detected by ELISA.Results Compared with normal control group,serum levels of TNF-αand IL-4 in model group statistically were significantly(P<0.05).Compared with normal control group,serum levels of TNF-αand IL-4 in sodium houttuyfonate group statistically were significantly(P<0.05).Compared with asthma group,serum levels of TNF-αand IL-4 in sodium houttuyfonate group statistically were significantly[(1198.7±261.6)ng/L,(36.2±2.34)pg/mL vs(1310.1±271.1)ng/L,(53.5±2.58)pg/mL](P<0.05).Conclusion Sodium houttuyfonate can effectively reduce the levels of TNF-αand IL-4 in serum of asthma mice.It suggested that is may treat asthma by inhibiting the activity of NF-κB of signal pathway by reduce the levels of TNF-αand IL-4 in mice.

关 键 词：鱼腥草素钠 哮喘 肿瘤坏死因子 白细胞介素-4 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]