镉对不同品种苜蓿种子萌发及幼苗生长的影响  

Effects of cadmium on seed germination and seedling growth of different alfalfa varieties

作　　者：保琼莉 唐一然 保万魁[3] 宋正国 黄益宗 BAO Qiong-li;TANG Yi-ran;BAO Wan-kui;SONG Zheng-guo;HUANG Yi-zong(Agro-Environmental Protection Institute,Ministry of Agriculture,Tianjing 300191,China;Tianjin Agricultural University,Tianjing 300392,China;Institute of Agricultural Resource and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]农业农村部环境保护科研监测所,天津300191 [2]天津农学院,天津300392 [3]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《生态学杂志》2020年第5期1695-1705,共11页Chinese Journal of Ecology

基　　金：国家重点研发计划项目课题(2017YFD0801502);国家自然科学基金项目(41807334);农业部环境保护科研监测所基本科研业务费专项(2019-jbkyywf-bql)资助。

摘　　要：通过培养皿滤纸萌发实验,研究了不同Cd2+浓度(0、5、10、20、50、100、200mg·L-1)对7种苜蓿种子萌发和幼苗生长的影响。结果表明,5~20 mg·L-1的Cd2+处理对不同品种苜蓿的萌发指标有不同程度的促进效应,10 mg·L-1的处理显著增加了甘农1号和阿尔冈金的鲜重。Cd2+对不同品种苜蓿萌发指标的抑制效应差异较大:Cd2+浓度5~200、20~200、50~200和200 mg·L-1的处理分别显著降低了皇后、牧歌、中兰1号和中兰2号的发芽势;50~200、100~200和200 mg·L-1的处理分别显著降低了中兰1号、牧歌(阿尔冈金)及中兰2号(皇后)的发芽率;50~200、100~200和200 mg·L-1的处理分别显著降低了中兰1号、牧歌(阿尔冈金)和中兰2号(皇后)的发芽指数;20~200 mg·L-1的处理均显著降低了所有品种苜蓿的活力指数;50~200 mg·L-1的处理均显著降低所有品种的鲜重,不同Cd2+浓度对7种苜蓿的根长和/或芽长均有显著抑制效应。所有萌发指标中,发芽率、发芽指数和鲜重与Cd2+浓度之间的Pearson相关性达到极显著水平,Cd浓度的变化对这些指标变化的解释量均超过83%。利用隶属函数值综合评价7种苜蓿对Cd2+的耐受性,皇后以其高的发芽率及Cd2+耐受性,可作为修复重金属污染土壤的优推品种。The effects of Cd2+with different concentrations(0,5,10,20,50,100,200 mg·L-1)on seed germination and seedling growth of seven alfalfa varieties were examined in a germination experiment with Petri dish filter paper.The results showed that germination indices of different alfalfa varieties were promoted by different extents with Cd2+treatments in the range of 5-20 mg·L-1.The fresh weight of Gannong No.1 and Algonquin was significantly increased under 10 mg·L-1 treatment.The inhibiting effects of Cd2+treatments on the examined indices of different alfalfa varieties were greatly different.The germination potential of Empress,Amerigraze,Zhonglan No.1 and Zhonglan No.2 was significantly decreased under 5-200,20-200,50-200 and 200 mg·L-1 treatments,respectively.The germination rates and germination indices of Zhonglan No.1,Algonquin(Amerigraze),and Zhonglan No.2(Empress)were significantly reduced under treatments with Cd2+concentration of 50-200,100-200 and 200 mg·L-1,respectively.Cd2+concentration of 20-200 mg·L-1 significantly decreased vigor index of all varieties.Fresh weight of all varieties significantly were decreased under treatments with Cd2+concentrations of 50-200 mg·L-1.All Cd2+treatments significantly inhibited root and/or bud length of seven varieties.The correlations between germination rate,germination index,freshweight and Cd2+concentration were significant,with R2 values being higher than 0.83.The tolerances of seven alfalfa varieties to Cd2+stress,as evaluated comprehensively using the average value of subordinate function of all measurement indices,followed an order of Gannong No.1>Empress>Zhonglan No.2>Tianshui Alfalfa>Algonquin>Amerigraze>Zhonglan No.1.In conclusion,the Empress,with high germination rate and tolerance to Cd2+stress,could be recommended as potential candidate variety for the remediation of cadmium contaminated soil.

关 键 词：镉 苜蓿 萌发 幼苗生长 

分 类 号：X503.231[环境科学与工程—环境工程] S541.9[农业科学—作物学]