中国部分地区猪胆汁戊型肝炎病毒基因型和中和抗原位点变异研究  

Genotypes and neutralizing antigen site variations of hepatitis E virus from swine bile in selected areas of China

作　　者：尹文娇[1] 韩雅琳 刘宏图[1] 姜法春 曹经瑗[1] 毕胜利[1] Yin Wenjiao;Han Yalin;Liu Hongtu;Jiang Fachun;Cao Jingyuan;Bi Shengli(NHC Key Laboratory of Medical Virology and Viral Diseases,National Institute f or Viral Disease Control and Prevention,Chinese Center f or Disease Control and Prevention,Bei jing 102206,China;Qingdao Municipal Center f or Disease Control and Prevention,Qingdao Institute of Preventive Medicine,Qingdao 266033,Shandong,China)

机构地区：[1]国家卫生健康委员会医学病毒和病毒病重点实验室,中国疾病预防控制中心病毒病预防控制所,北京102206 [2]青岛市疾病预防控制中心,青岛市预防医学研究院,山东青岛266033

出　　处：《中国疫苗和免疫》2020年第2期131-135,154,共6页Chinese Journal of Vaccines and Immunization

基　　金：国家科技重大专项(2018ZX10201002-009-002);侯云德院士科研青年基金(2019HYDQNJJ01)。

摘　　要：目的调查猪胆汁戊型肝炎病毒(HEV)携带率、基因型和中和抗原位点变异。方法在中国4个地区屠宰场采集成年商品猪的胆汁标本,采用real-time RT-PCR检测HEV RNA;采用巢式RT-PCR扩增HEV ORF2区部分基因片段,进行基因分型和中和抗原位点氨基酸序列分析。结果猪胆汁标本HEV RNA总阳性率为8.6%(46/532),4个地区阳性率在3.0%-12.5%之间。19株HEV基因分型成功,均为基因4型,其中11株HEV基因亚型分型成功,均为4a亚型。在该11株HEV的21个中和抗原位点中,2株HEV在ORF2区分别发生第487位氨基酸突变(Ser487Tyr)和第564位氨基酸突变(Thr564Pro)。结论中国部分地区猪胆汁HEV携带率较高,为中国人群中优势流行的基因4型,且部分中和抗原位点发生突变;应加强猪群HEV的持续监测。Objective To determine the carriage,genotypes and variations of neutralizing antigen sites of hepatitis E virus(HEV)in swine bile.Methods We collected bile samples of adult commercial swine from slaughterhouses of 4 areas in China to detect HEV RNA by real-time RT-PCR.We used nested RT-PCR to amplify parts of gene fragments of the ORF2 region for determining genotypes and analyzing amino acid sequences of neutralizing antigen sites.Results The overall positivity rate of HEV RNA was 8.6%(46/532)among swine bile samples,ranging from 3.0%to 12.5%in the 4 areas.Nineteen HEV strains were genotyped successfully and identified as genotype 4,and among these strains,11 were identified as sub-genotype4 a.Two of the 11 HEV strains had amino acid mutations at 2 of 21 neutralizing antigen sites of the ORF2 region-one at site 487(Ser487 Tyr)and the other at site 564(Thr564 Pro).Conclusions Carriage of HEV was high in swine bile in selected areas of China;isolated HEV was predominantly genotype 4,which has been circulating in Chinese people,with mutations in some neutralizing antigen sites.We should strengthen continuous monitoring of HEV in swine.

关 键 词：戊型肝炎病毒 基因型 中和抗原位点 变异 猪胆汁 

分 类 号：R183.4[医药卫生—流行病学]