水浸胁迫对超表达Sub1A油菜(Brassica napus)抗氧化能力的影响  

Influence of Water Immersion Stress on Antioxidant Capacity of Overexpressed Sub1A in Brassica napus L.

作　　者：苏良辰 周鹤峰 牛宪立 姬可平 Su Liangchen;Zhou Hefeng;Niu Xianli;Ji Keping(Department of Biological Engineering,Zhuhai Campus of Zunyi Medical University,Zhuhai,519041)

机构地区：[1]遵义医科大学珠海校区生物工程系,珠海519041

出　　处：《分子植物育种》2020年第10期3361-3367,共7页Molecular Plant Breeding

基　　金：贵州省科学技术基金项目(黔科合J字[2012]2347号)资助。

摘　　要：环境中水分含量变化对于生长期油菜的产量和品质影响极大。国内外研究表明,异源表达Sub1A基因具有提高水涝条件下植物的抗逆性和恢复生长的潜能。本研究以组成型表达Sub1A基因的甘蓝型油菜作为实验材料,测试了其在水浸胁迫30 d内的生长速度和抗氧化水平。结果表明,超表达株系在地上部分和地下部分的生物量积累速率均显著高于野生型油菜,在胁迫早期尤为明显,表现出响应水浸胁迫的优良表型。同时,抗氧化酶(SOD,POD,CAT和GPX)活性和胁迫响应基因的表达变化也基本一致,呈现出胁迫早期强,随着处理时间增加呈逐渐降低的趋势。以上结果说明超表达Sub1A基因可能通过提高细胞的抗氧化酶活性、提高胁迫响应基因的表达水平提升抗水浸胁迫能力。本研究将为深入研究外源表达Sub1A基因提高油菜抗水浸胁迫提供依据,并为抗涝油菜新品种的开发提供前期基础。The water content changing in environment has great influence on the yield and quality of Brassica napus.It has been shown in many studies that Sub1A gene could improve plant stress resistance and recovery ability under water immersion conditions at home and abroad.In this study,the constitutive expressed Sub1A gene Brassica napus lines were used as the experimental material to test the growth rate and antioxidant level within 30 d of water flooding stress.It was showed that the biomass accumulation of over-expressed lines in overground and underground parts were significantly higher than that of wild type.Meanwhile,the activity change of antioxidant enzymes(SOD,POD,CAT and GPX)and the expression change of stress-responsive genes were consistent,showing strong activity at the early stage of water stress and gradually decreased activity with treatment time.These results indicated that the overexpression of Sub1A gene may improve the stress resistance by increasing the activity of antioxidant enzymes in cells and the expression level of stress response genes.This study will provide a basis for further study on improving the stress resistance of Brassica napus by exogenously expressing Sub1A gene,and provide a preliminary basis for the development of new flooding stress resistant Brassica napus varieties.

关 键 词：Sub1A 油菜(Brassica NAPUS L.) 水浸胁迫 抗氧化 育种 

分 类 号：S565.4[农业科学—作物学]