野生何首乌组织培养研究  

The Tissue Culture of Wild Polygonum multiflorum

作　　者：农艳丰[1] 韦宗雪 李健[1] Nong Yanfeng(Baise University,Baise 533000,China)

机构地区：[1]百色学院,广西百色533000

出　　处：《安徽农学通报》2020年第10期15-17,71,共4页Anhui Agricultural Science Bulletin

基　　金：广西自然科学基金项目(2018GXNSFBA050026);广西高校中青年教师基础能力提升项目(2017KY0724);硕士学位授予单位立项建设授权点(桂学位[2018]7号)。

摘　　要：选用广西百色当地野生何首乌为试验材料,以茎段为外植体,探究其最适宜的组织培养方案。结果表明:75%乙醇30s配合0.1%HgCl2消毒12min,可明显控制外植体的污染率,是茎段分段灭菌中最适宜的方案;初代培养中,诱导腋芽选择MS+6-BA 2.0mg/L+KT 1.0mg/L+蔗糖30g/L+琼脂7g/L培养基进行培养,诱导率、总芽数及平均芽高均最高;无菌芽增殖时,添加50mL/L的椰汁配合6-BA 2.0mg/L,可得到最好的增殖率和芽数;生根培养以1/2MS+NAA 0.2mg/L+IBA 0.1mg/L为最适宜生根配方;高山土是野生何首乌组培苗适宜的移栽基质。In this study,the local wild Polygonum multiflorum in Guangxi was used as the research material,and the stems were used as explants to explore its most suitable tissue culture technology.The results showed that:75% etha⁃nol 30s with 0.1% HgCl2 disinfection for 12min could significantly control the contamination rate of explants,which was the most suitable scheme for segmental sterilization of stem segments;the selection of axillary buds in primary culture with MS+6-BA2.0mg/L+KT1.0mg/L+sucrose30g/L+agar 7g/L medium,which made the highest induction rate,total bud number and average bud height;6-BA 2.0mg/L was added during sterile bud proliferation,The best growth rate and number of shoots were obtained with coconut milk 50mL/L;rooting culture with 1/2MS+NAA 0.2mg/L+IBA 0.1 mg/L was the most suitable rooting formula.The alpine soil was a suitable transplanting substrate for the wild Po⁃lygonum multiflorum tissue culture seedlings.

关 键 词：何首乌 组织培养 茎段 

分 类 号：S567.239[农业科学—中草药栽培]