羌活胜湿汤的质量稳定与传递规律研究  

Study on Quality Stability and Transmission Law of Qianghuo Shengshitang

作　　者：梁慧慧 文雯 余格 毛妍 朱俊平[1,2] 肖美凤 刘文龙[1,2,4] 贺福元 张喜利[1,2] 刘瑞连 LIANG Hui-hui;WEN Wen;YU Ge;MAO Yan;ZHU Jun-ping;XIAO Mei-feng;LIU Wen-long;HE Fu-yuan;ZHANG Xi-li;LIU Rui-lian(Pharmacy College,Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Key Laboratory of Druggability and Preparation Modification for Traditional Chinese Medicine,Changsha 410208,China;Affiliated Hospital of Hunan Academy of Chinese Medicine,Changsha Hunan 410006,China;No.1 Traditional Chinese Medicine Hospital in Changde,Changde Hunan,415000,China)

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]中药成药性与制剂制备湖南省重点实验室,湖南长沙410208 [3]湖南省中医药研究院附属医院,湖南长沙410006 [4]湖南省常德市第一中医医院,湖南常德415000

出　　处：《时珍国医国药》2020年第1期75-78,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81874344);中国博士后基金项目(2018M640755);湖南省自然科学基金面上项目(2019JJ40220);湖南省长沙市科技局项目(kq1801034);湖南中医药大学研究生创新课题(2018CX52)。

摘　　要：目的建立羌活胜湿汤单味药及复方汤剂的HPLC指纹图谱,并采用总量统计矩法进行分析,探讨单味-复方过程中的质量稳定性和传递性规律。方法采用加热回流提取、水提醇沉对羌活胜湿汤复方及各单味药材进行处理,取复方及单味供试品液分别进行HPLC指纹图谱测定,通过总量统计矩加合参数法分析单味-复方过程中化学成分及其含量的稳定性与传递性规律。结果按处方中七单味药材指纹图谱数据计算得羌活胜湿汤复方的总量零阶矩、一阶矩、二阶矩分别为5.10×10^4 mAu·s,11.95 min,89.81 min^2,而实际测得的总方指纹图谱的总量零阶矩、一阶矩、二阶矩分别为6.49×10^4 mAu·s,15.23 min,115.79 min^2。结论单味-复方过程中质量属性较为稳定,其总体的传递性为78.20%。羌活胜湿汤复方中总物质基础信息为各单味药材传递而得,且各单味药的有效成分稳定的存在于复方汤剂中,单味-复方的成分群量值稳定且易传递,此规律或可用于中药制剂的质量监控分析。Objective To establish the HPLC fingerprint of single herb of Qianghuo Shengshitang and compound decoction,and to analyze the HPLC fingerprint by total statistical moment method for inquire the quality stability and transitivity law in the single-compound process.Methods Heat reflux extraction,water extraction and alcohol precipitation were used to process compound decoction and single herbs,HPLC fingerprints were used to determine the chemical constituents and their contents in the compound and single flavor solution.The stability and transmissibility of the chemical components and their contents in the process of single-compound were analyzed by the total statistical moment addition parameter method.Results According to the fingerprint data of seven single herbs in the prescription,the total zero moment,first moment and second moment of the compound of Qianghuo Shengshitang were 5.10×10^4 mAu·s,11.95 min,89.81 min^2,The total zero moment,first moment and second moment of the measured total square fingerprint are 6.49×10^4 mAu·s,15.23 min,115.79 min^2.Conclusion In the process of single-compound,the quality attribute is relatively stable,and its overall transitivity is 78.20%.The basic information of the total substances in Qianghuo Shengshitang was transmitted by each single herb,and the effective components of each single herb were stable in the compound decoction,and the group value of single-compound was stable and easy to transmit.This rule may be used for quality control and analysis of traditional Chinese medicine preparations.

关 键 词：羌活胜湿汤 总量统计矩 指纹图谱 质量属性 传递性规律 质量监控 

分 类 号：R283[医药卫生—中药学]