当归建中汤的高效液相色谱指纹图谱建立  

Establishment of HPLC Fingerprint of Danggui Jianzhong Decoction

作　　者：黎智辉 邓洁 赖华禄 丁新 刘秋杏 崔升淼 LI Zhihui;DENG Jie;LAI Hualu;DING Xin;LIU Qiuxing;CUI Shengmiao(College of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006 Guangdong,China)

机构地区：[1]广东药科大学中药学院,广东广州510006

出　　处：《中药新药与临床药理》2020年第6期708-713,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省科技计划项目(2013B031800019);广东药科大学重点培养教师计划项目。

摘　　要：目的建立当归建中汤标准汤剂的指纹图谱,为其质量评价提供方法及依据。方法制备10批次当归建中汤标准汤剂,采用高效液相色谱-二极管阵列检测器(HPLC-DAD)法对当归建中汤标准汤剂样品进行测定。色谱柱为Phenomenx Luna C18柱(250 mm×4.6 mm,5μm);柱温:30℃;流速:1 mL·min^-1;检测波长:254 nm;流动相为乙腈∶0.1%甲酸,梯度洗脱;进样量:10μL。结果建立了当归建中汤HPLC指纹图谱,确定了19个共有峰,指认了5个共有峰,分别为阿魏酸、芍药苷、苷草苷、甘草酸铵、肉桂酸;10批当归建中汤指纹图谱与对照图谱相似度均大于0.97。结论所建立指纹图谱方法灵敏度高、准确性好、有较高稳定性,能体现当归建中汤组分的整体特征,可为其质量控制提供依据。Objective To establish a fingerprint of the standard decoction of Danggui Jianzhong decoction(DJD),and provide a method and basis for the quality evaluation of DJD. Methods Ten batches of standard DJD were prepared. The detection of DJD was performed by HPLC-DAD method. The system used Phenomenx Luna C18 column(250 mm×4.6 mm,5 μm),column temperature was at 30 ℃,with the flow rate of 1 mL·min^-1,and detection wavelength at 254 nm,gradient elution with mobile phase consisted with acetonitrile and 0.1% formic acid,the injection volume was 10 μL. Results The HPLC fingerprint of DJD was established, in which 19 characteristic peaks were calibrated,and five characteristic peaks were determined. The similarities of fingerprints of 10 batches of DJD were all greater than 0.97. Conclusion The current fingerprinting method has high sensitivity,good accuracy and high stability,which can reflect the overall characteristics of DJD and provide a basis for the quality control of DJD.

关 键 词：当归建中汤 指纹图谱 高效液相色谱法 质量控制 

分 类 号：R284.1[医药卫生—中药学]