柏子仁及其炮制品对便秘小鼠肠道菌群的影响  

Effect of Arborvitae Seed(Platycladi Semen)and Its Processed Products on Intestinal Flora of Constipation Model Mice

作　　者：倪红辉[1] 吴育[1,2,3] 符映均 彭晓清 张硕[1] 王陈可汗 NI Hong-hui;WU Yu;FU Ying-jun;PENG Xiao-qing;ZHANG Shuo;WANG Chen-kehan(Nantong Traditional Chinese Medicine Hospital,Nantong Jiangsu 226001,China;College of Pharmacy,Nanjing University of Traditional Chinese Medicine,Nanjing Jiangsu 210023,China;Jiangsu Key Laboratory of Traditional Chinese Medicine Processing,Nanjing Jiangsu 210023,China;Animal Experimental Center,Nantong University,Nantong Jiangsu 226000,China)

机构地区：[1]南通市中医院,江苏南通226001 [2]南京中医药大学药学院,江苏南京210023 [3]江苏省中药炮制重点实验室,江苏南京210023 [4]南通大学动物实验中心,江苏南通226000

出　　处：《中医药导报》2020年第7期16-19,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：江苏省药学会-奥赛康医院药学科研项目(A201720);南通市市级科技项目(MS12017021-6)。

摘　　要：目的:探究生柏子仁及柏子仁霜对便秘模型小鼠肠道菌群的影响。方法:采用盐酸洛哌丁胺制造小鼠便秘模型,采用粪便含水量测定和小肠推进率验证小鼠便秘模型。利用16SrDNA宏基因组测序对便秘小鼠粪便中的菌群进行分析,研究生柏子仁、柏子仁霜对肠道菌群的影响。结果:生柏子仁组和柏子仁霜组对肠道菌群丰度产生影响,菌群科、属、种水平上相对丰度皆有差异。与模型组比较,生柏子仁组及柏子仁霜组菌种差异性均比较大的4种菌种是Candidatus_Saccharimonas、Enterorhabdus、Muribaculum、Ruminococcaceae_UCG-004。结论:生柏子仁和柏子仁霜缓解便秘可能与调节肠道菌群相关。Objective:To explore the effect of arborvitae seed(Platycladi Semen)and arborvitae seed frost(Platycladi Semen Pulveratum)on the intestinal flora of constipation model mice.Methods:Mice constipation model was established by loperamide hydrochloride,and the constipation model was verified by the determination of fecal water content and intestinal propulsion rate.The effect of arborvitae seed(Platycladi Semen)and arborvitae seed frost(Platycladi Semen Pulveratum)on intestinal flora in constipated mice was studied by 16SrDNA macro genome sequencing.Results:The relative abundance of intestinal flora in the two groups were different at the level of family,genus and species.Compared with the model group,the four species of bacterias with great difference between the arborvitae seed(Platycladi Semen)group and the arborvitae seed frost(Platycladi Semen Pulveratum)group were Candidatus_Saccharimonas,Enterorhabdus,Muribaculum,Ruminococcaceae_ucg-004.Conclusion:Arborvitae seed(Platycladi Semen)and arborvitae seed frost(Platycladi Semen Pulveratum)are closely related to relieving constipation and regulating intestinal flora.

关 键 词：生柏子仁 柏子仁霜 便秘 小鼠 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]