特克塞尔×哈萨克羊微卫星标记位点多态性与生产性状的关联分析  

Correlation between Polymorphism and Productive Traits of Texel × Kazakh Sheep Analyzed Using Microsatellite Markers

作　　者：李彬 李志强 韩冰 比拉力·艾山 张宁 刘明军 贺三刚[1] 依明·苏来曼[1] LI Bin;LI Zhi-qiang;HAN Bing;BILALI·Aishan;ZHANG Ning;LIU Ming-jun;HE San-gang;YIMING·Sulaiman(College of Animal Science,Xinjiang Agricultural University,Urumqi,Xinjiang 830052,China;Institute of Biotechnology,Xinjiang Academy of Animal Science/Key Laboratory of Genetic Breeding and Reproduction of Herbivorous Livestock,Ministry of Agriculture and Rural Affairs/Xinjiang Key Laboratory of Animal Biotechnology,Urumqi,Xinjiang 830011,China)

机构地区：[1]新疆农业大学动物科学学院,新疆乌鲁木齐830052 [2]新疆畜牧科学院生物技术研究所/农业农村部草食家畜遗传育种与繁殖重点实验室/新疆动物生物技术重点实验室,新疆乌鲁木齐830011

出　　处：《福建农业学报》2020年第3期243-253,共11页Fujian Journal of Agricultural Sciences

基　　金：新疆维吾尔自治区重点研发项目(2017B01005-1);新疆维吾尔自治区重点实验室开放课题(2018D04011);新疆维吾尔自治区天山创新团队计划项目(2018D14004)。

摘　　要：【目的】利用微卫星标记分析特克塞尔×哈萨克羊群体的遗传多样性,并与生产性状进行关联分析,为该品种的遗传育种和性状改良提供分子标记。【方法】选取11个微卫星标记,采用PCR扩增和毛细管电泳技术检测108只特克塞尔×哈萨克羊杂交群体的遗传多样性,并对各位点不同基因型与生产性状进行关联分析。【结果】11个标记位点在该群体中共检测到91个等位基因,平均等位基因数为8.273,平均观测杂合度为0.428,平均期望杂合度为0.815,平均多态信息含量为0.789。关联分析发现如下位点与相应性状之间呈极显著或显著相关:AMEL、ETH152、INRA006和INRA023与初生重、断奶重、宰前重和胴体重;CSRD247与胴体长、腿垂直长;INRA005、MAF214和MCM527与腿会斜长、腿耻斜长和踝骨宽;INRA172和OARFCB20位点与臀宽、胸宽和肩宽;INRA063与胸深。【结论】11个微卫星标记位点在该群体中具有较丰富的遗传多态性,且与生产性状均有不同程度的关联性,可以为特克塞尔×哈萨克羊性状改良和育种工作提供分子标记。【Objective】 Microsatellite markers were used to analyze the correlation between the genetic polymorphisms and productive traits of Texel×Kazakh sheep for breed improvement.【Method】 Eleven microsatellite marker sites were selected for the study. Genetic polymorphisms of 108 sheep were determined using PCR and capillary electrophoresis. Correlation between the genotypes and productive traits of the sheep at each site was analyzed.【 Result】 A total of 91 alleles were detected at 11 microsatellite loci with an averaged number of alleles at 8.273, an observed heterozygosity at 0.428, an expected heterozygosity at 0.815, and a content of polymorphic information at 0.789. The following loci were found extremely significantly or significantly correlate with their corresponding traits: AMEL, ETH152, INRA006 and INRA023 loci with primary weight, weaning weight, pre-mortem weight, and weight of the salamander;CSRD247 locus with lengths of carcass and legs;INRA005, MAF214 and MCM527 with leg will be obliquely long, the legs are obliquely long and the ankle width;INRA172 and OARFCB20 with widths of hip, chest and shoulder;and, INRA063 with chest depth.【Conclusion】 The 11 selected microsatellite markers were rich in genetic polymorphisms among the sheep population. They all correlated in different degrees with the various productive traits of a sheep suggesting a potential tool in breeding of Texel×Kazakh sheep with increased meat producing capacity.

关 键 词：特克塞尔×哈萨克羊 微卫星标记 遗传多样性 生产性状 

分 类 号：S813.22[农业科学—畜牧学]