优化绿色木霉发酵培养基提升真菌几丁质含量  

Enhancing chitin content in Trichoderma viride by medium optimization

作　　者：李晓鹏 叶金鹏 李振星 钱建瑛 丁振中 龚劲松 李恒 许正宏 史劲松 LI Xiaopeng;YE Jinpeng;LI Zhenxing;QIAN Jianying;DING Zhenzhong;GONG Jinsong;LI Heng;XU Zhenghong;SHI Jinsong(School of Pharmaceutical Sciences,Jiangnan University,Wuxi 214122,China;Agriculture and Rural Council of Ningjin County,Dezhou 253400,China;Yangzhou Rixing Bio-tech Co.,Ltd.,Yangzhou 225600,China)

机构地区：[1]江南大学药学院,江苏无锡214122 [2]宁津县农业农村局,山东德州253400 [3]扬州日兴生物科技股份有限公司,江苏扬州225600

出　　处：《生物加工过程》2020年第3期283-288,共6页Chinese Journal of Bioprocess Engineering

基　　金：江苏省科技成果转化专项(BA2015006)。

摘　　要：几丁质是N乙酰葡糖胺通过β1,4糖苷键连接聚合而成的结构多糖,在医药、食品、纺织、环保等领域具有重要应用价值。以金针菇菌渣为基料,开展绿色木霉的培养基配方优化及发酵试验,以提高真菌中几丁质含量。结果表明:绿色木霉菌的最适碳源、氮源分别为葡萄糖30 g/L和硫酸铵4 g/L,金针菇菌渣100 g/L,在初始pH为6.0、培养基装液量为50 mL(250 mL三角瓶)、发酵周期为3 d时,几丁质含量达到最高(13%),质量浓度可达到2.65 g/L。本结果为低成本、工业化生产几丁质提供了科学依据。Chitin is a structural polysaccharide formed by the polymerization of N-acetylglucosamine byβ-1,4-glycosidic linkage.It has shown great application potential in pharmacy,food,textile and environmental protection.We optimized the culture medium for Trichoderma viride with spent substrate of Flammulina velutipes as the basic raw material,to increase the chitin content.The optimum ingredientsare medium compostion was 30 g/L glucose,4 g/L ammonium sulphate,and 100 g/L spent substrate of Flammulina velutipes.We obtained the highest content of chitin of 13%(titer of 2.65 g/L)in the 3rd day of the fermentation,with initial pH of 6 and medium volume of 50 mL in a 250-mL flask.This study provides scientific support to commercially producing chitin in a low-cost way.

关 键 词：几丁质 金针菇菌渣 绿色木霉 培养基优化 废弃物再利用 

分 类 号：Q93[生物学—微生物学]