NaCl和等渗PEG胁迫对桑树叶片光系统Ⅱ的影响  

Effect of NaCl and PEG Iso-osmotic Stress on PhotosystemⅡin Mulbery Leaves

作　　者：陈泉印 徐宗艺 王月 孙广玉[1] CHEN Quanyin;XU Zongyi;WANG Yue;SUN Guangyu(College of Life Science,Northeast Forestry University,Harbin,Heilongjiang 150040)

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《北方园艺》2020年第7期92-100,共9页Northern Horticulture

基　　金：国家自然科学基金资助项目(31870373)。

摘　　要：以桑树品种"秋雨桑"为试材,利用植物叶绿素荧光动力学技术,研究了等渗NaCl和PEG胁迫对桑树叶片光合作用的光系统Ⅱ(PSⅡ)的影响。结果表明:与对照(CK)比较,桑树叶片在150 mmol·L-1 NaCl和等渗17.1%PEG胁迫下均保持较高的PSⅡ反应中心活性,具有完善的光防御保护机制。寻找NaCl和等渗PEG对桑树叶片PSⅡ反应中心的伤害部位,发现4种抑制剂对2种胁迫条件下桑树叶片的光合作用位点不同,盐胁迫主要通过叶黄素循环途径,以及维持QA、QB间电子的正常传递来保护PSⅡ反应中心活性,而PEG胁迫下D1蛋白周转减弱伤害。Taking mulberry(Morus alba L.)as the experimental material,the effect of mulberry leaves on photosystemⅡ(PSⅡ)photoinhibition under salt stress and isotonic PEG stress was investigated by utilizing inhibitor.The results showed that compared with CK,mulberry leaves maintained higher PSⅡreaction center activity under 150 mmol·L-1 NaCl and isotonic 17.1%PEG stress,and had perfect light damage protection mechanism.Under the simulation of salt and isotonic drought stress,the damage of mulberry leaf which happened in PSⅡreaction centers by utilizing inhibitor.The photosynthetic sites of mulberry leaves were different using two kinds of stress conditions after under inhibitors.Salt stress mainly protected PSⅡreaction center activity through lutein cycle pathway and normal electron transfer between QAand QB,while D1 protein turnover played a greater role under PEG stress.

关 键 词：桑树 NACL胁迫 PEG胁迫 D1蛋白周转 叶黄素循环 光合PSⅡ 

分 类 号：S945.1[农业科学—水产养殖]