支气管哮喘患者肝X受体、蛋白磷酸酶1A的表达及与气道重塑的关系研究  

Expression of liver X receptors and protein phosphatase 1A and their relationship with airway remodeling in bronchial asthma patients

作　　者：郑琳[1] 王丽[2] 毛山[2] 周丹阳[2] 谷伟[2] 史莹[2] ZHENG Lin;WANG Li;MAO Shan;ZHOU Danyang;GU Wei;SHI Ying(Department of Thoracic and Cardiovascular Surgery,Nanjing First Hospital,Nanjing Medical University,Nanjing,Jiangsu 210006,P.R.China;Department of Respiratory and Critical Care Medicine,Nanjing First Hospital,Nanjing Medical University,Nanjing,Jiangsu 210006,P.R.China)

机构地区：[1]南京医科大学附属南京医院(南京市第一医院)心胸外科,江苏南京210006 [2]南京医科大学附属南京医院(南京市第一医院)呼吸与危重症医学科,江苏南京210006

出　　处：《中国呼吸与危重监护杂志》2020年第3期229-233,共5页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：南京市医学科技发展项目;南京市卫生计委杰出青年基金(JQX16028)。

摘　　要：目的评估支气管哮喘患者气道重塑水平,并检测患者外周血肝X受体(LXRs)、蛋白磷酸酶1A(PPM1A)、转化生长因子-β1(TGF-β1)、Smad2的表达水平,确定LXRs和PPM1A是否与气道重塑相关。方法受试者分健康对照组、轻中度哮喘组和重症哮喘组,各30例。对支气管哮喘患者进行肺功能及高分辨率CT检查,评估气道重塑情况。抽取外周血,离心后检测血清中LXRα、LXRβ、PPM1A、TGF-β1和Smad2,进行统计学分析。结果轻中度哮喘组的气道重塑水平明显高于对照组,重症哮喘组的气道重塑水平明显高于轻中度哮喘组。哮喘组血清LXRα、LXRβ的水平较对照组增高,重症哮喘组的LXRα与LXRβ水平较轻中度哮喘组增高,与气道重塑指标无明显相关性。轻中度哮喘组血清PPM1A水平较对照组降低,重症哮喘组的PPM1A水平较轻中度哮喘组降低,且PPM1A水平与气道重塑存在负相关。结论哮喘患者PPM1A水平较健康者减低,PPM1A水平与气道重塑程度负相关。哮喘患者血清LXRs较健康者增高,与气道重塑程度无明显相关。Objective To detect the expression of Liver X receptors(LXRs),protein phosphatase 1A(PPM1A),transforming growth factor-β1(TGF-β1)and Smad2 in peripheral blood of bronchial asthma patients,and to explore whether LXRs and PPM1A are related to airway remodeling.Methods Subjects were divided into healthy control group,mild and moderate asthma group and severe asthma group,with 30 subjects each.Lung function and high-resolution computed tomography examination were performed on patients with bronchial asthma to define airway remodeling.Peripheral blood was extracted and the serum levels of LXRα,LXRβ,PPM1A,TGF-β1 and Smad2 were detected after centrifugation.Then the data were analyzed.Results The airway remodeling level of the mild and moderate asthma group was significantly higher than that of the control group.The airway remodeling level of the severe asthma group was significantly higher than that of the mild and moderate asthma group.Serum LXRα,LXRβin asthma group were higher than those in the control group.The levels of LXRαand LXRβin severe asthma were higher than those in mild and moderate asthma group.There was no significant correlation between LXRs and airway remodeling.The PPM1A level in mild and moderate asthma group was lower than that in the control group.The levels of PPM1A in severe asthma were lower than that in mild and moderate asthma group.PPM1A level was negatively correlated with airway remodeling.Conclusions The level of PPM1A in asthma patients is lower than that in healthy subjects,and is negatively correlated with the degree of airway remodeling.Serum LXRs in asthmatic patients are higher than that in healthy subjects,but LXRs are not significantly correlated with airway remodeling.

关 键 词：哮喘 肝X受体 蛋白磷酸酶1A 

分 类 号：R562.25[医药卫生—呼吸系统]