双氢杨梅树皮素对博来霉素诱导小鼠肺纤维化的影响  

Effect of ampelopsin in mice with bleomycin-induced pulmonary fibrosis

作　　者：黄海涛 方静 曾春晖 银锡靖 洪正善 谭柳萍 杨柯 HUANG Haitao;FANF Jing;ZENG Chunhui;YIN Xijing;HONG Zhengshan;TAN Liuping;YANG Ke(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning,Guangxi 530200,P.R.China;Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development,Nanning,Guangxi 530020,P.R.China)

机构地区：[1]广西中医药大学药学院,广西南宁530020 [2]广西优势中成药与民族药开发工程技术中心,广西南宁530020

出　　处：《中国呼吸与危重监护杂志》2020年第3期270-275,共6页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：广西科技厅项目(11-031-05-Z1);广西教育厅广西研究生教育创新计划资助项目(JGY2014086);广西教育厅研究生教育创新课题计划资助项目(YCSZ2018002);广西中医药大学杨世林教授团队人才培养建设项目(CS17017)。

摘　　要：目的通过体内模型研究双氢杨梅树皮素的抗肺纤维化作用,为寻找抗肺纤维化药物奠定基础。方法将60只BALB/c小鼠按体重随机数表法随机分为6组,每组10只,即生理盐水空白对照组、博来霉素模型组(3 mg/kg)、地塞米松组(2.5 mg/kg),以及双氢杨梅树皮素不同剂量干预组(200、100、50 mg/kg)。除空白对照组外,各组以博来霉素为诱导剂诱导BALB/c小鼠肺纤维化模型,采用无创气管内滴注法肺部滴注博来霉素溶液(3 mg/kg)分别于第1 d和第14 d各滴注1次,实验周期为28 d。各干预组在模型构建期间分别使用地塞米松(2.5 mg/kg)与双氢杨梅树皮素(200、100、50 mg/kg)进行干预,空白对照组与模型组同法给予生理盐水。28 d后分别测定小鼠各项指标,肺功能测定仪测定呼吸功能(吸气阻力、肺动态顺应性、每分钟最大通气量),计算肺脏器系数,测定肺组织羟脯氨酸水平,并进行肺组织病理学检查。结果模型组小鼠呼吸功能较空白对照组显著下降(P<0.05),药物高剂量干预能显著改善呼吸功能(P<0.05);模型组小鼠肺脏器系数显著增加(P<0.05),其余各组肺脏器系数均显著下降(P<0.05);模型组肺部羟脯氨酸水平显著升高(P<0.05),而药物干预组羟脯氨酸含量显著降低(P<0.05);模型组小鼠肺泡壁显著增厚,胶原纤维显著增加,肺泡炎症与纤维化评分明显升高(P<0.05),而药物干预组症状减轻。结论双氢杨梅树皮素能抵抗由博来霉素诱导的小鼠肺纤维化,改善呼吸功能衰退的症状,减少胶原蛋白的形成,产生抗小鼠肺纤维化的作用。Objective This study construct a pulmonary fibrosis model in vivo to study anti-pulmonary fibrosis effect of ampelopsis.Methods We constructed a pulmonary fibrosis model by bleomycin in BALB/c mice.The mice were divided by weight random number table into a blank control group,a model control group,a dexamethasone treatment group(intervened with dexamethasone in a dose of 2.5 mg/kg),and three ampelopsis treatment groups intervened with ampelopsis in dose of 200,100,and 50 mg/kg,respectively.Bleomycin solution(3 mg/kg)was intratracheally injected respectively on 1st and 14th day,except the blank group.Twenty-eight days later,the relevant indicators were collected,including respiratory function(airway resistance,dynamic lung compliance,maximal ventilator volume),level of hydroxyproline and histopathological changes in the lungs.Results After 28 days,the model control group mice had severe respiratory resistance,dynamic lung compliance and maximal ventilator volume were decreased.The high dose ampelopsis treatment could enhance respiratory function(P<0.05).Lung coefficient was lower in the treatment groups than that in the model control group(P<0.05).The hydroxyproline of the treatment groups was less than that of the model control group(P<0.05).Histopathological examination showed that the degree of fibrosis increased in the model control group(P<0.05),but decreased in the treatment groups(P<0.05).Conclusion Ampelopsis can resist bleomycin-induced pulmonary fibrosis in mice,relieve the symptoms of respiratory failure,reduce the formation of collagen,and produce anti-pulmonary fibrosis effect.

关 键 词：肺纤维化 双氢杨梅树皮素 呼吸功能 体内实验 博来霉素 

分 类 号：R285.5[医药卫生—中药学]