烤烟不同部位叶片超微结构与常规化学成分的关系  

Ultrastructural differences of flue-cured tobacco from different stalk positions and their relationship with chemical components

作　　者：夏春 李寒雪 孙觅 曹明锋 刘园 刘欢 雷晓 何佶弦 景延秋[5] 程玉渊 杨益芬 XIA Chun;LI Hanxue;SUN Mi;CAO Mingfeng;LIU Yuan;LIU Huan;LEI Xiao;HE Jixian;JING Yanqiu;CHENG Yuyuan;YANG Yifen(Sichuan Branch of China Tobacco Corporation,Chengdu,Sichuan 610000,China;Henan Zhongyan Industry Co.Ltd,Zhengzhou,Henan 450002,China;Changde Branch of Hunan Tobacco Corporation,Changde,Hunan 415000,China;Nanyang Branch of Henan Tobacco Corporation,Nanyang,Henan 473000,China;College of Tobacco,Henan Agricultural University,Zhengzhou,Henan 450002,China;Chengdu Customs Technology Co.Ltd Technology Center,Chengdu,Sichuan 610041,China)

机构地区：[1]中国烟草总公司四川省公司,四川成都610000 [2]河南中烟工业有限责任公司,河南郑州450002 [3]湖南省烟草公司常德市公司,湖南常德415000 [4]河南省烟草公司南阳市公司,河南南阳473000 [5]河南农业大学烟草学院,河南郑州450002 [6]成都海关技术中心,四川成都610041

出　　处：《湖南农业大学学报（自然科学版）》2020年第2期150-156,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：四川省烟草公司泸州市公司项目(202051050024019);中国烟草总公司四川省烟草公司项目(2019410102000453);河南中烟工业有限责任公司项目(2019410001340094);湖南省烟草公司常德市公司项目(2018430700240157);河南省烟草公司南阳市公司项目(2018411301240127);四川省烟草公司广元市公司项目(202051080024063)。

摘　　要：采用数字量化图像方法对烤烟不同部位叶片细胞超微结构进行量化,分析细胞面积、细胞周长、细胞形状因子、液泡面积、细胞壁厚度、液泡面积与细胞面积比值与烤烟化学成分的关系。结果表明:烤烟叶片表面细胞轮廓清晰,细胞壁内侧均有细胞核、叶绿体、线粒体等贴壁分布,中央大液泡填充于细胞中间;烤烟上部叶细胞面积、液泡面积、周长较小,细胞壁厚度较大;中部叶细胞面积、液泡面积、周长、细胞壁厚度较小;下部叶液泡面积、细胞壁厚度、液泡面积与细胞面积比值较大;烤烟叶片细胞壁厚度与蛋白质、氯含量呈正相关,与总糖含量呈负相关;细胞面积、细胞周长、细胞形状因子与烟碱、总氮含量呈正相关;细胞面积、液泡面积与还原糖含量呈正相关;细胞形状因子、液泡面积与细胞面积比值和蛋白质含量呈负相关。The cell ultrastructural images of different parts of flue-cured tobacco were quantified by digital quantitative image method, the relationship between cell area, cell perimeter, cell shape factor, vacuole area, cell wall thickness, ratio of vacuole area to cell area and chemical composition index of flue-cured tobacco quality was analyzed. The results showed that the cell outline on the surface of flue-cured tobacco leaves was clear, there were obviously cell nucleus, chloroplast, mitochondria and other adherent distribution on the inner side of cell wall, and the central large vacuole filled the cells in the middle. The cell area, vacuole area and cell perimeter in the upper leaves of flue-cured tobacco were small, and the cell wall thickness was large;the cell area, vacuole area, cell perimeter and cell wall thickness in the middle leaves were small;the vacuole area, cell wall thickness and the ratio of vacuole area to cell area in the lower leaves was large. The thickness of cell wall is positively correlated with the contents of protein and chlorine, and negatively correlated with the content of total sugar;the cell area, cell perimeter, and cell shape factor are positively correlated with the contents of nicotine and total nitrogen;the cell area, vacuole area are positively correlated with the content of reducing sugar;the cell shape factor and the ratio of vacuole area to cell area are negatively correlated with protein content.

关 键 词：烤烟 叶片细胞 超微结构 量化 化学成分 

分 类 号：S572.01[农业科学—烟草工业]