基于流式细胞术的桫椤基因组大小测定  

The genome size determination of Alsophila spinulosa based on flow cytometry

作　　者：黄雄 郭英华 田林 李全梓 岳志强[1] HUANG Xiong;GUO Yinghua;TIAN Lin;LI Quanzi;YUE Zhiqiang(State Key Laboratory of Tree Genetics and Breeding,Chinese Academy of Forestry,Beijing 100091,China)

机构地区：[1]中国林业科学研究院林木遗传育种国家重点实验室,北京100091

出　　处：《湖南农业大学学报（自然科学版）》2020年第2期171-175,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：中国林业科学研究院基本科研业务费专项资金项目(CAFYBB2017ZY001)。

摘　　要：以买麻藤(Gnetum montanum)为内参,测定5种常用裂解液(Tris·MgCl2、LB01、WPB、Otto’s、Galbraith’s)对桫椤(Alsophila spinulosa)细胞核的裂解效果,并通过比较桫椤和买麻藤流式细胞仪测定的荧光峰值,计算出桫椤的基因组大小。结果表明:5种裂解液中,WPB为桫椤最佳裂解液;桫椤基因组大小为6 003.25Mb,即2C DNA含量为6.138 pg。In this study, to find out the optimal lysis buffer for dissociating cell nucleus, five common lysis buffers(Tris·MgCl2, LB01, WPB, Otto’s, Galbraith’s) were tested on the cell nucleus of Alsophila spinulosa leaves by use of Gnetum montanum as internal standard, and, the genome size of A. spinulosa was calculated according to the multiple relationships of peak values of A. spinulosa and Gnetum montanum using flow cytometry. The results showed that the WPB was the most appropriate lysis buffer to dissociate A. spinulosa cell nucleus and the genome size of A. spinulosa was determined to be 6 003.25 Mb, i.e. 2C DNA content was 6.138 pg.

关 键 词：桫椤 买麻藤 裂解液 流式细胞术 基因组大小 

分 类 号：Q943.2[生物学—植物学]