苯丙氨酸和茉莉酸甲酯对龙眼胚性悬浮细胞柯里拉京积累的影响  

Effects of phenylalanine and methyl jasm onate on the growth and corilagin accum ulation of em bryogenic suspension cells in Dimocarpus longan Lour.

作　　者：廖斌 徐小萍[1] 李珊珊 梁梓豪 李汉生[1] 林玉玲[1] 赖钟雄[1] LIAO Bin;XU Xiaoping;LI Shanshan;LIANG Zihao;LI Hansheng;LIN Yuling;LAI Zhongxiong(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学园艺植物生物工程研究所,福州350002

出　　处：《应用与环境生物学报》2020年第2期287-293,共7页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(31572088);国家级大学生创新创业训练项目(201710389019);福建省重大科技专项专题(2015NZ0002-1);科技创新专项基金(CXZX2017189)资助。

摘　　要：基于龙眼胚性悬浮细胞体系的建立,研究苯丙氨酸(Phe)和茉莉酸甲酯(MeJA)不同添加浓度处理对龙眼胚性悬浮细胞生长和柯里拉京积累的影响.在龙眼细胞培养第6天添加苯丙氨酸和茉莉酸甲酯不同浓度处理,测定龙眼细胞干重、柯里拉京含量、苯丙氨酸解氨酶(PAL)和多酚氧化酶(PPO)活性,并利用qPCR技术分析柯里拉京合成相关基因DlDFR、DlLAR和DlANR的表达模式.结果显示,适宜浓度的苯丙氨酸和茉莉酸甲酯能促进龙眼胚性悬浮细胞的生长和柯里拉京的积累,最适添加浓度分别为20 mg/L和50μmol/L,与对照组相比,柯里拉京含量分别提高了1.9倍、2.9倍,产量分别提高了4.9倍、6.8倍;苯丙氨酸和茉莉酸甲酯能诱导PAL和PPO活性提高,并可能通过诱导柯里拉京生物合成途径上DlDFR、DlLAR、DlANR基因表达下调,进而调控龙眼胚性悬浮细胞中柯里拉京的合成与积累.本研究表明适宜浓度的苯丙氨酸和茉莉酸甲酯可诱导PAL和PPO活性提高,有效促进龙眼胚性悬浮细胞中柯里拉京含量的积累,结果可为今后龙眼细胞大规模培养工业化生产柯里拉京提供科学依据和理论指导.(图4表1参46)The effects of different concentrations of phenylalanine(Phe)and methyl jasmonate(MeJA)on the growth and corilagin accumulation of embryogenic suspension cells in longan were examined in this study.Based on the establishment of an embryonic suspension culture system,the longan cells were treated with different concentrations of Phe and MeJA on the 6th day of the suspension culture,after which the dry weight of longan cells,corilagin content,and phenylalanine lyase(PAL)and polyphenol oxidase(PPO)activity were determined.The expression patterns of the genes related to corilagin synthesis DlDFR,DlLAR,and DlANR were analyzed using real-time polymerase chain reaction.The results showed that appropriate Phe and MeJA concentrations promoted the growth of embryogenic suspension cells of longan and corilagin accumulation,with the optimum concentrations being 20 mg/L and 50μmol/L,respectively.Compared with the control group,the corilagin content was increased by 1.9 times and 2.9 times,and production was increased by 4.9 times and 6.8 times,respectively.Furthermore,higher PAL and PPO activities were induced by Phe and MeJA,and the synthesis and accumulation of corilagin in longan cells were regulated by the downregulated expression of DlDFR,DlLAR,and DlANR genes in the corilagin biosynthesis pathway.In conclusion,appropriate concentrations of Phe and MeJA increased PAL and PPO activities,and effectively promoted the accumulation of corilagin,which provide a scientific basis and theoretical guidance for the large-scale cultivation of longan cells and industrialized production of corilagin.

关 键 词：龙眼 胚性悬浮细胞 苯丙氨酸 茉莉酸甲酯 柯里拉京 

分 类 号：S667.2[农业科学—果树学]