基于共轭物的酸性水解——利用萃取-衍生化-GC/MS分析人尿中17-酮类固醇  

Based on acidic hydrolysis of conjugates--Analysis of 17-ketosteroids in Human Urine by Extraction-Derivation-GC/MS Analysis

作　　者：高原 Gao Yuan(Criminal Investigation Police University of China,Shenyang 110854,China)

机构地区：[1]中国刑事警察学院,辽宁沈阳110854

出　　处：《山东化工》2020年第8期121-123,共3页Shandong Chemical Industry

摘　　要：本实验建立萃取-衍生化-GC/MS对人尿中17-酮类固醇(17-KS)分析的方法。首先,优化了17-KS共轭物在人尿中的酸水解条件。随后,使用含有HFB氯化物的萃取溶剂来应用七氟代丁酰(HFB)衍生化方法。结果表明:用3%HCl在100℃加热20min,使17-KS最大产率释放出。当使用含有5%HFB-氯化物的正己烷作为萃取溶剂时,回收率最高。同时该方法可以在室温下同时衍生和提取17-KS。因此,该方法在法庭科学中可用于鉴别人的尿液。This experiment established a method for extraction-derivative-GC/MS analysis of 17-ketosteroids(KS)in human urine.First,the acid hydrolysis conditions of 17-KS conjugates in human urine were optimiz-ed.Subsequently,extraction solvents containing HFB chlorides were used to apply sevoflubutyryl(HFB)deri-vatization methods.The results showed that the 17-KS maximum yield was released by heating with 3%H-Cl at 100℃for 20min.When n-hexane containing 5%HFB-chloride was used as the extraction solvent,the recovery was the highest.At the same time,17-KS can be derived and extracted simultaneously at room tem-perature.Therefore,this method can be used in court science to identify human urine.

关 键 词：酸性水解 衍生化 GC-MS 17-KS 

分 类 号：D918.4[政治法律—法学] R87[医药卫生—运动医学]