美花石斛和铁皮石斛试管苗物质含量比较  

Comparative Study on Content of Test Tube Seedlings of Dendrobium loddigesii Rolfe and Dendrobium officinale Kimura et Migo

作　　者：曾宇馨 张悦 祝天添 贺雨馨 孙志蓉[1] ZENG Yu-xin;ZHANG Yue;ZHU Tian-tian;HE Yu-xin;SUN Zhi-rong(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《中国现代中药》2020年第4期577-584,共8页Modern Chinese Medicine

基　　金：现代农业产业技术体系建设专项(CARS-21)。

摘　　要：目的:研究比较美花石斛试管苗和铁皮石斛试管苗物质组分差异,揭示有效物质动态累积与生长规律的关系,为石斛培养提供参数支持。方法:观测2种石斛不同时期生长发育情况,用HPLC测定糖类、氨基酸及内源激素含量,进行比较分析。结果:两者动态积累生物量趋势基本一致;多糖/总糖值均较大,美花石斛多糖含量较高,铁皮石斛单糖种类多且含量高;氨基酸总量均呈降低趋势;赤霉素及玉米素含量差异有统计学意义,生长素及脱落酸变化趋势一致。美花石斛赤霉素含量与苗高、根长及干物质积累速率呈正相关,生长素含量与分蘖数呈负相关;赤霉素/脱落酸值高利于植株生长,生长素/玉米素值低可促进分蘖发生。结论:铁皮石斛进入生物量快速增长期较美花石斛早,且持续时间长。铁皮石斛生长量高于美花石斛是由于多时期同时测到多种糖类。美花石斛氨基酸总量高于铁皮石斛,说明美花石斛营养价值更高。美花石斛赤霉素低而脱落酸高、赤霉素/脱落酸及生长素/玉米素低可能是导致生长慢、纤弱及分蘖多的重要原因。Objective:To study the differences in material composition between Dendrobium loddigesii Rolfe and D.officinale Kimura et Migo,and to reveal the relationship between the dynamic accumulation of effective substances and the growth law,and provide parameter support for Dendrobium culture.Method:The growth and development of two species of Dendrobium were observed in different periods.The contents of sugar,amino acid and endogenous hormone were determined by HPLC.Results:The trend of dynamic accumulation of biomass in both is basically the same.The polysaccharide/total sugar value was larger,the polysaccharide content of D.loddigesii Rolfe was higher,and the single sugar content of D.officinale Kimura et Migo was high and the content was high.The total amount of amino acids in both groups showed a decreasing trend.The GA3 and ZR contents of the two were significantly different,and the trends of IAA and ABA were consistent.For D.loddigesii Rolfe,there was a positive correlation between GA3 content and seedling height,root length and dry matter accumulation rate.IAA content was negatively correlated with tiller number;GA3/ABA value was high for plant growth,and low IAA/ZR value could promote tillering.Conclusion:D.officinale Kimura et Migo enters the period of rapid growth of biomass,which is earlier than the D.loddigesii Rolfe,and lasts for a long time.The growth of D.officinale Kimura et Migo is higher than that of Rhododendron chinensis because of the simultaneous determination of various sugars in multiple periods.The total amino acid content of D.loddigesii Rolfe higher than that of D.officinale Kimura et Migo,indicating that the nutritional value of D.loddigesii Rolfe higher.Low GA3 and high ABA,low GA3/ABA and IAA/ZR may be important reasons for slow growth,weakness and tillering.

关 键 词：美花石斛 铁皮石斛 试管苗 生长特性 物质组分 

分 类 号：R282[医药卫生—中药学]