不同栽培方式产地蒙古黄芪中黄芪甲苷和总黄酮含量差异分析  

Difference Analysis of AstragalosideⅣand Total Flavonoids Content from Astragalus membranaceus var.mongolicus under Different Cultivation Patterns and Producing Areas

作　　者：田文仓 冯紫薇 石福娟 石玥 张靖晗 丁一明 栾震 宋学武 赵婷[1,4] 魏胜利[1,4] TIAN Wen-cang;FENG Zi-wei;SHI Fu-juan;SHI Yue;ZHANG Jing-han;DING Yi-ming;LUAN Zhen;SONG Xue-wu;ZHAO Ting;WEI Sheng-li(College of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Zizhou County Industrial Development Investment Co.,Ltd.,Yulin 718400,China;Management Center for Drug Delivery,National Health Commission of People′s Republic of China,Beijing 102488,China;Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs,Ministry of Education,Beijing 100102,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]子洲县产业发展投资有限公司,陕西榆林718400 [3]中华人民共和国国家卫生健康委员会药具管理中心,北京102488 [4]中药材规范化生产教育部工程研究中心,北京100102

出　　处：《中国现代中药》2020年第4期585-590,602,共7页Modern Chinese Medicine

基　　金：北京中医药大学自主课题(2019-JYB-XS-070)。

摘　　要：目的:比较不同栽培方式、产地蒙古黄芪中黄芪甲苷、总黄酮含量差异。方法:采用HPLC、紫外分光光度法分别测定黄芪甲苷、总黄酮含量并对其结果进行方差分析。结果:育苗移栽和种子直播蒙古黄芪中黄芪甲苷平均质量分数分别为0.151%、0.245%,总黄酮平均质量分数分别为0.508%、0.541%。不同产地移栽蒙古黄芪中黄芪甲苷质量分数为0.061%~0.323%,总黄酮质量分数为0.376%~0.903%;不同产地种子直播蒙古黄芪中黄芪甲苷质量分数为0.161%~0.343%,总黄酮质量分数为0.399%~0.825%。结论:不同栽培方式的蒙古黄芪中黄芪甲苷含量差异有统计学意义(P<0.05),且种子直播黄芪大于育苗移栽黄芪,总黄酮含量差异无统计学意义;不同产地蒙古黄芪中黄芪甲苷和总黄酮含量差异有统计学意义。其中,育苗移栽蒙古黄芪中山西朔州市产区的黄芪甲苷含量最高,宁夏河川乡产区所含总黄酮含量最高。种子直播蒙古黄芪中,陕西子洲县西庄乡产区的黄芪甲苷含量最高,陕西府谷县产区的黄芪总黄酮含量最高。Objective:To compare the difference of astragalosideⅣand total flavonoids content in Astragalus membranaceus var.mongolicus under different cultivation patterns and producing areas.Methods:The content of astragalosideⅣand total flavonoids were determined by HPLC and UV spectrophotometry,and the results were analyzed by variance.Results:The average content of astragalosideⅣin the seedling transplanted and direct-seeded A.membranaceus var.mongolicus were 0.151%and 0.245%,respectively.And the average content of total flavonoids were 0.508%and 0.541%,respectively.The content of astragalosideⅣin seedling transplanted A.membranaceus var.mongolicus was between 0.061%and 0.323%,and the total flavonoids content was between 0.376%and 0.903%.The content of astragalosideⅣin the direct-seeded A.membranaceus var.mongolicus of different producing areas was between 0.161%and 0.343%,and the total flavonoids content was between 0.399%and 0.825%.Conclusion:The content of astragalosideⅣin A.membranaceus var.mongolicus under different cultivation patterns is significantly different(P<0.05),and the direct-seeded A.membranaceus var.mongolicus is larger than the seedling transplanted A.membranaceus var.mongolicus However,there is no significant difference in total flavonoids.The content of astragalosideⅣand total flavonoids in A.membranaceus var.mongolicus.under different producing areas are significantly different.Among them,the content of astragalosideⅣin seedling transplanted A.membranaceus var.mongolicus produced in Shuozhou of Shanxi was the highest,and the total flavonoids content of A.membranaceus var.mongolicus produced in Hechuan of Ningxia was the highest.The content of astragalosideⅣin direct-seeded A.membranaceus var.mongolicus produced in Xizhuang Township,Zizhou,Shaanxi was the highest,and the total flavonoids content of A.membranaceus var.mongolicus produced in Fugu,Shaanxi was the highest.

关 键 词：蒙古黄芪 栽培方式 不同产地 黄芪甲苷 总黄酮 

分 类 号：R282[医药卫生—中药学]