大气颗粒物PM2.5对老年雄性大鼠骨代谢的影响  

Effects of atmospheric particulate matter PM2.5 on bone metabolism in aged male rats

作　　者：张宝玉[1] 尹瑞梨 莫彩艳 赵冬[1] 马燕[1] 柯静 ZHANG Baoyu;YIN Ruili;MO Caiyan;ZHAO Dong;MA Yan;KE Jing(Center for Endocrine Metabolism and Immune Diseases,Beijing Luhe Hospital,Capital Medical University,Beijing,100149,China)

机构地区：[1]首都医科大学附属北京潞河医院内分泌代谢与免疫性疾病中心,北京100149

出　　处：《中国医药导报》2020年第15期4-7,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(81800723);北京市通州区科技计划项目潞河医院首都临床特色应用研究项目(KJ2019CX012-51)。

摘　　要：目的观察大气细颗粒物PM2.5对老年雄性大鼠骨密度、骨小梁结构的影响,及对核因子κ受体激活剂Β配体(RANKL)/骨保护素(OPG)表达的作用,探讨PM2.5对老年大鼠骨代谢的影响。方法采用雾霾仓暴露系统,按照随机数字表法将20只19月龄老年SD雄性大鼠分为清洁组和PM2.5组,每组10只。将其分别饲养于清洁仓和雾霾仓,每周监测体重,12周后,取材留取股骨组织,检测大鼠骨密度。苏木精-伊红(HE)染色观察骨组织形态;提取骨髓中的mRNA,应用Real-time PCR检测RANKL/OPG的表达情况。结果两组体重随着时间的延长呈现稳定的增高,但两组体重比较,差异无统计学意义(P>0.05)。HE结果显示,与清洁组比较,PM2.5组松质骨部位的骨小梁减少,排列稀疏、部分断裂。与清洁组比较,PM2.5组骨密度、骨小梁数量、骨小梁面积和骨小梁面积百分比均明显下降,差异均有统计学意义(P<0.05或P<0.01)。Real-time PCR结果显示,与清洁组比较,PM2.5组骨髓中的OPG mRNA含量降低,而RANKL mRNA含量增高,差异均有统计学意义(均P<0.05)。结论长期暴露于PM2.5中有可能通过增加成骨细胞RANKL表达并降低OPG表达,增加破骨细胞活性,进而降低骨密度和骨小梁,加重老年大鼠骨质疏松。Objective To observe the effect of atmospheric fine particulate matter PM2.5 on bone mineral density and trabecular structure of aged male rats,and the effect of receptor activator of nuclear factor-kappa B ligand(RANKL)/osteoprotegerin(OPG)expression of function,and to explore PM2.5 effect on bone metabolism of aged rats.Methods A total of 20 male SD rats aged 19 months were divided into the cleaning group and the PM2.5 group according to the random number table method using a haze warehouse exposure system,with 10 rats in each group.They were kept in a clean warehouse and a haze warehouse respectively,and their weight was monitored weekly.After 12 weeks,femoral tissue was collected and bone mineral density of rats was detected.Bone morphology was observed by hematoxylin-eosin(HE)staining.The mRNA in bone marrow was extracted and the expression of RANKL/OPG was detected by Real-time PCR.Results The body weight of the two groups increased steadily with the extension of time,but there was no signifant difference in body weight between the two groups(P>0.05).HE results showed that compared with the cleaning group,the bone trabeculae in the cancellous bone of the PM2.5 group were reduced,arranged sparsely and partially fractured.Compared with the clean group,bone mineral density,trabecular number,trabecular area and percentage of trabecular area in the PM2.5 group all decreased significantly,with statistically significant differences(P<0.05 or P<0.01).Real-time PCR results showed that compared with the clean group,OPG mRNA content in the PM2.5 group was decreased,while RANKL mRNA content was increased,with statistically significant differences(all P<0.05).Conclusion Long-term exposure to PM2.5 may increase osteoclast activity by increasing the expression of osteoblasts RANKL and decreasing the expression of OPG,thereby reducing bone mineral density and bone trabeculae and aggravating osteoporosis in aged rats.

关 键 词：骨代谢 PM2.5 大鼠 核因子κ受体激活剂B配体 骨保护素 

分 类 号：R2[医药卫生—中医学]