柳树新品种指纹图谱构建  

Construction of fingerprint for new Salix varieties

作　　者：郑纪伟[1,2] 教忠意 王保松[1,2] 何旭东 Zheng Jiwei;Jiao Zhongyi;Wang Baosong;He Xudong(Jiangsu Academy of Forestry, Nanjing 211135, China;Willow Nursery of the Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 211135, China)

机构地区：[1]江苏省林业科学研究院,江苏南京211153 [2]江苏省农业种质资源保护与利用平台柳树资源圃,江苏南京211153

出　　处：《江苏林业科技》2020年第2期1-5,56,共6页Journal of Jiangsu Forestry Science & Technology

基　　金：江苏省林业科学研究院青年基金项目“基于DNA条形码技术的柳树分子鉴定研究”(JAF-2016-01);江苏省林业科技创新与推广项目“抗逆速生灌木柳新品种区域性试验与示范”(LYKJ[2019]43);江苏省林业科学研究院自主创新项目“柳树叶绿体全基因组测序和亲缘关系的鉴定”(BM2018022-1)。

摘　　要：该研究利用荧光SSR标记构建了9个柳树新品种的指纹图谱。11对SSR标记共检测到56条等位片段,每个位点等位基因数3—8不等,平均为5.1个。观测杂合度(Ho)变化范围为0.3333—1.0000,平均为0.6875;期望杂合度(He)变化范围为0.4771—0.8824,平均为0.6263;多态信息量(PIC)变化范围为0.4212—0.8134,平均为0.6059。优选的3对核心引物中,SALeSSR0086与SALeSSR0733组合、SALeSSR0086与SALeSSR0920组合可完全区分9个柳树新品种。聚类结果显示,9个柳树新品种遗传相似系数在0.6059与0.9107之间。该研究建立的荧光SSR基因分型体系高效、准确,不仅能为柳树品种鉴定和新品种保护提供科学理论依据,也能为柳树进一步育种工作奠定坚实的基础。The fingerprint of 9 Salix varieties was established by fluorescent SSR marker in this study.56 alleles were detected by 11 pairs of SSR markers from Salix,and the number of alleles per locus ranged from 3 to 8,with an average of 5.1 alleles.The observed heterozygosity(Ho),the expected heterozygosity(He)and the polymorphic information content(PIC)varied from 0.3333—1.0000(mean 0.6875),0.4771—0.8824(mean 0.6263)and 0.4212—0.8134(mean 0.6059),respectively.Among the 3 optimal pairs of core primers,SALeSSR0086 and SALeSSR0733 combinations,SALeSSR0086 and SALeSSR0920 combinations could completely distinguish 9 Salix varieties.The cluster analysis showed that the similarity coefficient of the 9 Salix varieties ranged from 0.6059—0.9107,and the different varieties from the same parents could be clustered in the same branch.The fluorescence SSR genotyping system established in this study was efficient,rapid and accurate,which could not only provide scientific theoretical basis for the identification of varieties and the protection of new Salix varieties,but also provide a solid foundation for the further Salix breeding.

关 键 词：柳树 荧光标记 SSR 指纹图谱 构建 

分 类 号：Q755[生物学—分子生物学] S792.12[农业科学—林木遗传育种]